Kim Yun-Mi, Lee Jin-A, Jung Bock-Gie, Kim Tae-Hoon, Lee Bong-Joo, Suh Guk-Hyun
Department of Veterinary Infectious Disease, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
Center for Pulmonary and Infectious Disease Control, University of Texas Health Science Center at Tyler, Tyler, TX, USA.
Anim Sci J. 2016 Jun;87(6):796-801. doi: 10.1111/asj.12485. Epub 2015 Sep 30.
There are no accurate reference ranges for hematology parameters and lymphocyte subsets in Korean native beef cattle (Hanwoo). This study was performed to establish reliable reference ranges of hematology and lymphocyte subsets using a large number of Hanwoo cattle (n = 350) and to compare differences between Hanwoo and Holstein dairy cattle (n = 334). Additionally, age-related changes in lymphocyte subsets were studied. Bovine leukocyte subpopulation analysis was performed using mono or dual color flow cytometry. The leukocyte subpopulations investigated in healthy cattle included: CD2(+) cells, sIgM(+) cells, MHC class II(+) cells, CD3(+) CD4(+) cells, CD3(+) CD8(+) cells, and WC1(+) cells. Although Hanwoo and Holstein cattle are the same species, results showed several differences in hematology and lymphocyte subsets between Hanwoo and Holstein cattle. This study is the first report to establish reference ranges of hematology and lymphocyte subsets in adult Hanwoo cattle.
韩牛（韩国本土肉牛）的血液学参数和淋巴细胞亚群没有准确的参考范围。本研究旨在通过大量韩牛（n = 350）建立可靠的血液学和淋巴细胞亚群参考范围，并比较韩牛与荷斯坦奶牛（n = 334）之间的差异。此外，还研究了淋巴细胞亚群的年龄相关变化。使用单色或双色流式细胞术进行牛白细胞亚群分析。在健康牛中研究的白细胞亚群包括：CD2(+)细胞、sIgM(+)细胞、MHC II类(+)细胞、CD3(+) CD4(+)细胞、CD3(+) CD8(+)细胞和WC1(+)细胞。尽管韩牛和荷斯坦牛属于同一物种，但结果显示韩牛和荷斯坦牛在血液学和淋巴细胞亚群方面存在一些差异。本研究是首次报道建立成年韩牛血液学和淋巴细胞亚群参考范围。
