George Jeanne W, Snipes Joy, Lane V Michael
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis, Davis, CA 95616, USA.
Vet Clin Pathol. 2010 Jun;39(2):138-48. doi: 10.1111/j.1939-165X.2009.00208.x. Epub 2010 Jan 7.
The most commonly used bovine hematology reference intervals were published in 1965. We found the results from healthy cattle in 2001 differed from those in many ways. Discovery of the original laboratory book used to calculate the 1965 values gave us the opportunity to evaluate whether hematology values of healthy cattle have changed over time.
The purpose of this study was to establish hematology reference intervals for Holstein cows, compare selected hematologic results with similar population data from 1957, and compare these reference intervals with those of other North American veterinary schools and published values.
Reference intervals were developed in 2001 using clinically healthy, bovine leukemia virus-negative, mid-lactation Holstein cows. Selected parts of the hemograms and neutrophil:lymphocyte (N:L) ratio were compared with those from healthy, age-matched Holstein cows evaluated in 1957. Bovine reference intervals were solicited from clinical pathology laboratories in North American veterinary colleges and analyzed for population characteristics and method of analysis.
Between 1957 and 2001, mean neutrophil counts increased significantly, whereas lymphocyte, monocyte, and eosinophil counts and hemoglobin concentration decreased significantly. Mean N:L ratio increased significantly to 1.17. Most surveyed laboratories were using the 1965 reference intervals. Two other institutions that had developed reference intervals after 2000 had results similar to ours.
Continued use of older bovine hematology reference intervals could lead to misinterpretation of within-reference neutrophil counts as neutrophilia and under-recognition of neutropenia, eosinophilia, monocytosis, or lymphocytosis. Use of N:L>1 as evidence of inflammation should be discontinued or used with great caution.
最常用的牛血液学参考区间于1965年发布。我们发现2001年健康牛的检测结果在很多方面与之不同。找到用于计算1965年参考值的原始实验室记录，让我们有机会评估健康牛的血液学值是否随时间发生了变化。
本研究的目的是建立荷斯坦奶牛的血液学参考区间，将选定的血液学结果与1957年类似群体的数据进行比较，并将这些参考区间与其他北美兽医学院的参考区间及已发表的值进行比较。
2001年，使用临床健康、牛白血病病毒阴性、处于泌乳中期的荷斯坦奶牛制定参考区间。将血细胞计数图的选定部分以及中性粒细胞与淋巴细胞比例（N:L）与1957年评估的健康、年龄匹配的荷斯坦奶牛的相应指标进行比较。向北美兽医学院的临床病理实验室征集牛参考区间，并分析其群体特征和分析方法。
1957年至2001年期间，中性粒细胞平均计数显著增加，而淋巴细胞、单核细胞、嗜酸性粒细胞计数以及血红蛋白浓度显著下降。平均N:L比例显著增加至1.17。大多数接受调查的实验室使用的是1965年的参考区间。另外两个在2000年之后制定参考区间的机构得到了与我们相似的结果。
继续使用旧的牛血液学参考区间可能导致将参考范围内的中性粒细胞计数误判为中性粒细胞增多，并对中性粒细胞减少、嗜酸性粒细胞增多、单核细胞增多或淋巴细胞增多认识不足。应停止使用或极其谨慎地使用N:L>1作为炎症证据。
