Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Mol Neurobiol. 2016 Oct;53(8):5278-87. doi: 10.1007/s12035-015-9442-5. Epub 2015 Sep 29.
Human endometrial and bone marrow-derived mesenchymal stem cells can be differentiated into a number of cell lineages. Mesenchymal stem cells (MSCs) are potential candidates for cellular therapy. The differentiation of human bone marrow MSCs (hBM-MSCs) and endometrial stem cells (hEnSCs) into motor neuron-like cells has been rarely investigated previously; however, the comparison between these stem cells when they are differentiated into motor neuron-like cell is yet to be studied. The aim of this study was therefore to investigate and compare the capability of hBM-MSCs and hEnSCs cultured on tissue culture polystyrene (TCP) and poly ε-caprolactone (PCL) nanofibrous scaffold to differentiate into motor neuron-like cells in the presence of neural inductive molecules. Engineered hBM-MSCs and hEnSCs seeded on PCL nanofibrous scaffold were differentiated into beta-tubulin III, islet-1, Neurofilament-H (NF-H), HB9, Pax6, and choactase-positive motor neurons by immunostaining and real-time PCR, in response to the signaling molecules. The data obtained from PCR and immunostaining showed that the expression of motor neuron markers of both hBM-MSCs and hEnSCs differentiated cells on PCL scaffold are significantly higher than that of the control group. The expression of these markers in hEnSCs differentiated cells was higher than that in hBM-MSCs. However, this difference was not statistically significant. In conclusion, differentiated hBM-MSCs and hEnSCs on PCL can provide a suitable three-dimensional situation for neuronal survival and outgrowth for regeneration of the central nervous system. Both cells may be potential candidates for cellular therapy in motor neuron disorders. However, differentiation of hEnSCs into motor neuron-like cells was better than hBM-MSCs.
人子宫内膜和骨髓间充质干细胞可分化为多种细胞谱系。间充质干细胞（MSCs）是细胞治疗的潜在候选者。人骨髓间充质干细胞（hBM-MSCs）和子宫内膜干细胞（hEnSCs）向运动神经元样细胞的分化以前很少被研究过；然而，这些干细胞在分化为运动神经元样细胞时的比较尚未被研究。因此，本研究旨在研究和比较在神经诱导分子存在的情况下，在组织培养聚苯乙烯（TCP）和聚己内酯（PCL）纳米纤维支架上培养的 hBM-MSCs 和 hEnSCs 分化为运动神经元样细胞的能力。通过免疫染色和实时 PCR ，将工程化的 hBM-MSCs 和 hEnSCs 接种在 PCL 纳米纤维支架上，分化为β-微管蛋白 III、胰岛 1、神经丝-H（NF-H）、HB9、Pax6 和胆碱酯酶阳性运动神经元，对信号分子做出响应。从 PCR 和免疫染色获得的数据表明，hBM-MSCs 和 hEnSCs 分化细胞在 PCL 支架上的运动神经元标志物的表达均明显高于对照组。hEnSCs 分化细胞的这些标志物的表达高于 hBM-MSCs。然而，这种差异没有统计学意义。总之，PCL 上分化的 hBM-MSCs 和 hEnSCs 可以为中枢神经系统的再生提供一个适合神经元存活和生长的三维环境。这两种细胞都可能是运动神经元疾病细胞治疗的潜在候选者。然而，hEnSCs 向运动神经元样细胞的分化要好于 hBM-MSCs。
