Jahani Hoda, Jalilian Farid Azizi, Wu Chia-Yu, Kaviani Saeid, Soleimani Masoud, Abbasi Naghmeh, Ou Keng-Liang, Hosseinkhani Hossein
Department of Physiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
J Biomed Mater Res A. 2015 May;103(5):1875-81. doi: 10.1002/jbm.a.35328. Epub 2014 Sep 24.
Differentiation of mesenchymal stem cells (MSCs) into neuron cells has great potential in therapy of damaged nerve tissue. It has been shown that three-dimensional biomaterials have great ability to up regulate the expression of neuronal proteins. In this study, O2 plasma technology was used to enhance hydrophilicity of poly (ε-caprolactone) (PCL) toward selective differentiation of MSCs into neural cells. Random and aligned PCL nanofibers scaffolds were fabricated by electrospinning method and their physicochemical and mechanical properties were carried out by scanning electron microscope (SEM), contact angle, and tensile measurements. Contact angle studies of PCL and plasma treated PCL (p-PCL) nanofibers revealed significant change on the surface properties PCL nanofibers from the view point of hydrophilicity. Physiochemical studies revealed that p-PCL nanofibers were extremely hydrophilic compared with untreated PCL nanofibers which were highly hydrophobic and nonabsorbent to water. Differentiation of MSCs were carried out by inducing growth factors including basic fibroblast growth factor, nerve growth factor, and brain derived growth factor, NT3, 3-isobutyl-1-methylxanthine (IBMX) in Dulbecco's modified Eagle's medium/F12 media. Differentiated MSCs on nanofibrous scaffold were examined by immunofluorescence assay and was found to express the neuronal proteins; β-tubulin III and Map2, on day 15 after cell culture. The real-time polymerase chain reaction (RT-PCR) analysis showed that p-PCL nanofibrous scaffold could upregulate expression of Map-2 and downregulate expression of Nestin genes in nerve cells differentiated from MSCs. This study indicates that mesenchymal stem cell cultured on nanofibrous scaffold have potential differentiation to neuronal cells on and could apply in nerve tissue repair.
间充质干细胞（MSCs）向神经细胞的分化在受损神经组织治疗中具有巨大潜力。研究表明，三维生物材料具有强大的上调神经元蛋白表达的能力。在本研究中，采用O2等离子体技术增强聚（ε-己内酯）（PCL）的亲水性，以促进MSCs向神经细胞的选择性分化。通过静电纺丝法制备了随机排列和定向排列的PCL纳米纤维支架，并通过扫描电子显微镜（SEM）、接触角和拉伸测量对其物理化学和力学性能进行了研究。PCL和经等离子体处理的PCL（p-PCL）纳米纤维的接触角研究表明，从亲水性角度来看，PCL纳米纤维的表面性质发生了显著变化。物理化学研究表明，与高度疏水且不吸水的未处理PCL纳米纤维相比，p-PCL纳米纤维具有极强的亲水性。在杜氏改良伊格尔培养基/F12培养基中，通过诱导生长因子，包括碱性成纤维细胞生长因子、神经生长因子、脑源性生长因子、NT3、3-异丁基-1-甲基黄嘌呤（IBMX）来实现MSCs的分化。通过免疫荧光分析检测了纳米纤维支架上分化的MSCs，发现在细胞培养15天后表达神经元蛋白β-微管蛋白III和Map2。实时聚合酶链反应（RT-PCR）分析表明，p-PCL纳米纤维支架可上调MSCs分化的神经细胞中Map-2的表达并下调巢蛋白基因的表达。本研究表明，在纳米纤维支架上培养的间充质干细胞具有向神经元细胞分化的潜力，可应用于神经组织修复。
