Arya Ridhima, Sharma Anil K
Department of Biotechnology, M.M.E.C., M.M. University, Mullana-Ambala -133207, India.
Curr Pharm Biotechnol. 2015;17(2):185-9. doi: 10.2174/1389201016666150930115737.
Excessive use of pesticides in agriculture has resulted in contamination of water resources, air, soil and disruption of biogeochemical cycles. These compounds adversely affect humans and animal health, helpful soil microbes and crop production as well. Biodegradation of pesticides by microbes exists in a number of habitats like soil, sediments, surface, ground water, and sludge, etc. In the present study, efforts were made to develop a microbial consortium comprising of Streptomyces albogriseolus and Brevibacillus borstelensis strains isolated earlier which are capable of degrading carbendazim, a benzimidazole fungicide and making it harmless. Both the strains Brevibacillus borstelensis and Streptomyces albogriseolus displayed growth even at higher concentrations (500μg mL(-1)) of carbendazim. The consortium containing Brevibacillus borstelensis and Streptomyces albogriseolus reduced carbendazim concentration from 30 µg mL(-1) to 0.86 µg mL(-1) (nearly 97%) in 12hrs to 0.60 µg mL(-1) (~98%) in 20 hrs as determined by LCMS analysis. There was a significant reduction observed in carbendazim concentration than reduction obtained when individual strain was used. This study paves a way for further exploration of degradation mechanism at the genetic level to enhance the capability of microorganisms in consortia.
农业中过度使用农药已导致水资源、空气、土壤受到污染，并破坏了生物地球化学循环。这些化合物对人类和动物健康、有益的土壤微生物以及作物生产均产生不利影响。微生物对农药的生物降解存在于许多生境中，如土壤、沉积物、地表水、地下水和污泥等。在本研究中，我们努力构建一个微生物联合体，该联合体由先前分离出的白灰链霉菌和博斯特伦短芽孢杆菌菌株组成，它们能够降解多菌灵（一种苯并咪唑类杀菌剂）并使其无害化。博斯特伦短芽孢杆菌和白灰链霉菌这两种菌株即使在较高浓度（500μg mL(-1)）的多菌灵环境下仍能生长。通过液相色谱 - 质谱联用（LCMS）分析测定，含有博斯特伦短芽孢杆菌和白灰链霉菌的联合体在12小时内将多菌灵浓度从30 µg mL(-)1降至0.86 µg mL(-1)（近97%），在20小时内降至0.60 µg mL(-1)（约98%）。与单独使用单个菌株时相比，多菌灵浓度有显著降低。本研究为在基因水平上进一步探索降解机制以提高联合体中微生物的能力铺平了道路。
