Department of Microbiology, Key Lab of Environmental Microbiology for Agriculture, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
College of Resource and Environment, Anhui Science and Technology University, Fengyang 233100, China.
J Hazard Mater. 2021 Jul 15;414:125496. doi: 10.1016/j.jhazmat.2021.125496. Epub 2021 Feb 24.
As a widely used fungicide, the environmental fate of carbendazim and its residues in agricultural products have caused great concern. However, its effects on soil microbial communities are largely unknown. Herein, we used high-throughput sequencing to reveal the effects of high and low dose of carbendazim and its degrading strain, Rhodococcus qingshengii strain djl-6, on the composition, diversity, and interrelationship of soil bacterial and fungal communities in short- and medium-term under laboratory conditions. The results showed that carbendazim exhibited an increased negative impact on bacterial communities and reduced the proportion of dominant fungal phylum Ascomycota during a 14-day incubation period. Only the impacts of low-dose carbendazim (2 mg·kg dry soil) on fungal community were weakened. Network analysis showed that carbendazim increased the connectivity and modularity of microbial co-occurrence networks. Strain djl-6 exhibited good potential for bioremediation of carbendazim-contaminated soils. Moreover, it driven the assembly of potential carbendazim-degrading consortia from indigenous microbial communities; and members of the genera Arthrobacter, Bacillus, Brevundimonas, Lysinibacillus, Massilia, Mycobacterium, Paenibacillus, and Pseudarthrobacter might be participated in the degradation of carbendazim. Taken together, our study provides a relatively comprehensive understanding of the effects of carbendazim and its degrading strain djl-6 on soil microbial communities.
多菌灵作为一种广泛使用的杀菌剂，其在农产品中的环境归宿及其残留问题引起了极大关注。然而，其对土壤微生物群落的影响在很大程度上尚不清楚。在此，我们采用高通量测序技术，揭示了高、低剂量多菌灵及其降解菌 Rhodococcus qingshengii 菌株 djl-6 对实验室短期和中期土壤细菌和真菌群落组成、多样性及相互关系的影响。结果表明，在 14 天的培养期内，多菌灵对细菌群落表现出更强的负向影响，降低了优势真菌门子囊菌门的比例。只有低剂量多菌灵（2 mg·kg 干土）对真菌群落的影响减弱。网络分析表明，多菌灵增加了微生物共发生网络的连接性和模块性。菌株 djl-6 对多菌灵污染土壤具有良好的生物修复潜力。此外，它驱动了潜在多菌灵降解菌丛从土著微生物群落中的组装；节杆菌属、芽孢杆菌属、短小芽孢杆菌属、赖氨酸芽孢杆菌属、马塞利亚菌属、分枝杆菌属、芽孢杆菌属和假节杆菌属的成员可能参与了多菌灵的降解。总之，本研究为多菌灵及其降解菌 djl-6 对土壤微生物群落的影响提供了较为全面的认识。
