Young C L, Frank H, Stewart C R, Wainer I W
Pharmaceutical Division, St. Jude Children's Research Hospital, Memphis, Tennessee 38105.
Chirality. 1989;1(3):235-8. doi: 10.1002/chir.530010309.
An enantioselective gas chromatographic method has been developed and validated for the determination of the plasma concentration of the enantiomers of the anticancer drug ifosfamide (IFF). In this approach, the IFF enantiomers are separated from the plasma matrix by solid phase extraction, chromatographically resolved by gas chromatography on a chiral stationary phase, and detected by mass selective detection using selective ion monitoring. The assay has been validated for routine clinical and pharmacokinetic use and has a limit of detection in plasma of 250 ng/ml of each isomer.
已开发并验证了一种对映体选择性气相色谱法，用于测定抗癌药物异环磷酰胺（IFF）对映体的血浆浓度。在该方法中，通过固相萃取将IFF对映体与血浆基质分离，在手性固定相上通过气相色谱进行色谱分离，并使用选择性离子监测通过质量选择性检测进行检测。该测定法已针对常规临床和药代动力学用途进行了验证，血浆中每种异构体的检测限为250 ng/ml。
