Wang Qian, Wang Chuan-Dong, Li Cheng-Hou, Li Jun-Gang, Chen Qi, Li Yue-Zhong
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
School of Life Science and Biotechnology, Mianyang Normal University, Mianyang 621000, PR China.
Int J Syst Evol Microbiol. 2015 Dec;65(12):4730-4733. doi: 10.1099/ijsem.0.000641. Epub 2015 Sep 28.
A strictly anaerobic, Gram-stain-positive bacterium, designated FW431T, was isolated from a mud cellar used for producing strong aromatic Chinese liquors. The strain was able to produce butanoic acid, an important component of the aroma style of Chinese liquors. Cells of strain FW431T were straight or slightly curved rods with a polar endospore and peritrichous flagella. The major cellular fatty acids (>10 % of the total) were C16 : 0, C18 : 1ω9c and C18 : 0. Biolog assays indicated that the strain preferably metabolizes palatinose, l-fucose, β-hydroxybutyric acid, l-rhamnose and α-ketobutyric acid among 95 carbon sources tested. FW431T was related most closely to Clostridium ljungdahlii DSM 13528T and Clostridium kluyveri DSM 555T based on 16S rRNA gene sequence similarities of 95.0 and 94.2 %, respectively. The DNA G+C content of the genomic DNA was 44.4 mol%. Based on the evidence presented here, FW431T ( = CGMCC 1.5201T = KCTC 15519T) is proposed as the type strain of a novel species, Clostridium luticellarii sp. nov.
从用于生产浓香型白酒的老窖泥中分离出一株严格厌氧、革兰氏染色阳性的细菌，命名为FW431T。该菌株能够产生丁酸，这是中国白酒香气风格的一种重要成分。FW431T菌株的细胞为直杆状或稍弯曲的杆状，有极生芽孢和周生鞭毛。主要的细胞脂肪酸（占总量的>10%）为C16:0、C18:1ω9c和C18:0。Biolog分析表明，在测试的95种碳源中，该菌株优先代谢帕拉金糖、L-岩藻糖、β-羟基丁酸、L-鼠李糖和α-酮丁酸。基于16S rRNA基因序列相似性分别为95.0%和94.2%，FW431T与Ljungdahlii梭菌DSM 13528T和Kluyveri梭菌DSM 555T关系最为密切。基因组DNA的G+C含量为44.4mol%。根据此处提供的证据，建议将FW431T（=CGMCC 1.5201T=KCTC 15519T）作为一个新物种——泥窖梭菌（Clostridium luticellarii sp. nov.）的模式菌株。
