衰老过程中自然杀伤细胞表型和功能的流式细胞术分析

Flow Cytometry Analysis of NK Cell Phenotype and Function in Aging.

作者信息

Tarazona Raquel, Campos Carmen, Pera Alejandra, Sanchez-Correa Beatriz, Solana Rafael

机构信息

Immunology Unit, Department of Physiology, University of Extremadura, Caceres, Spain.

Department of Immunology, IMIBIC, Reina Sofia University Hospital, University of Cordoba, Cordoba, 14071, Spain.

出版信息

Methods Mol Biol. 2015;1343:9-18. doi: 10.1007/978-1-4939-2963-4_2.

DOI:10.1007/978-1-4939-2963-4_2

PMID:26420705

Abstract

Natural killer (NK) cells represent a subpopulation of lymphocytes involved in innate immunity, defined recently as group 1 of innate lymphoid cells (ILCs). NK cells are cytotoxic lymphocytes with a relevant role in the destruction of transformed cells as virus-infected or tumor cells, as well as the regulation of the immune response through cytokine and chemokine production that activates other cellular components of innate and adaptive immunity. In humans, NK cell subsets have been defined according to the level of expression of CD56. Aging differentially affects NK cell subsets and NK cell function. Here, we describe protocols for the delineation of NK cell subsets and the analysis of their functional capacity using multiparametric flow cytometry.

摘要

自然杀伤（NK）细胞是参与固有免疫的淋巴细胞亚群，最近被定义为固有淋巴细胞（ILC）1组。NK细胞是细胞毒性淋巴细胞，在破坏转化细胞（如病毒感染细胞或肿瘤细胞）以及通过产生细胞因子和趋化因子调节免疫反应方面发挥重要作用，这些细胞因子和趋化因子可激活固有免疫和适应性免疫的其他细胞成分。在人类中，NK细胞亚群已根据CD56的表达水平进行定义。衰老对NK细胞亚群和NK细胞功能的影响存在差异。在此，我们描述了使用多参数流式细胞术描绘NK细胞亚群及其功能能力分析的方案。