Philip Joseph, Kushwaha Neerja, Chatterjee Tathagata, Mallhi Rajiv Singh
Department of Transfusion Medicine, AFMC, Pune, Maharashtra, India.
Asian J Transfus Sci. 2015 Jul-Dec;9(2):163-7. doi: 10.4103/0973-6247.162714.
As processing and cryopreservation of cord blood is time consuming and costly, it is essential to select units with optimal CD34+ cells, total nucleated cell (TNC) number and colony forming units (CFUs). These are the most important factors affecting outcome of UCB transplantation and are influenced by various maternal and neonatal factors.
To determine the maternal and neonatal factors affecting TNC and CD34+ cell counts in cord blood so as to aid in proper selection of cord blood units for cryopreservation.
A total of 100 UCB units were collected from normal vaginal deliveries, processed and assessed for volume, TNC, CD34+ cell count and CFU-GM. These parameters were then analyzed to find out whether they correlated with maternal and neonatal characteristics such as mother's age, parity, gestational age, baby's birth weight, and sex.
The volume of CB collected significantly correlated with the TNC, CD34+ cell, and CFU-GM yields (P < 0.02). A heavier placenta (P < 0.05), and a heavier baby (P < 0.002) were associated with a significantly greater volume of CB whereas the age, parity of mother and the sex of the baby had no significant effect.
The only factors found to affect the TNC and CD34+ cell counts significantly were weight of the baby and placenta and the volume of cord blood collected. Since these factors are of prognostic significance, their analysis will aid in deciding which UCB unit should be processed and cryopreserved for UCB banking and subsequent transplantation.
由于脐带血的处理和冷冻保存既耗时又昂贵，因此选择具有最佳CD34+细胞、总核细胞（TNC）数量和集落形成单位（CFU）的单位至关重要。这些是影响脐血移植结果的最重要因素，并受各种母体和新生儿因素的影响。
确定影响脐带血中TNC和CD34+细胞计数的母体和新生儿因素，以帮助正确选择用于冷冻保存的脐带血单位。
从正常阴道分娩中收集了总共100个脐血单位，对其进行处理并评估体积、TNC、CD34+细胞计数和CFU-GM。然后分析这些参数，以确定它们是否与母体和新生儿特征相关，如母亲年龄、产次、孕周、婴儿出生体重和性别。
收集的脐血体积与TNC、CD34+细胞和CFU-GM产量显著相关（P<0.02）。较重的胎盘（P<0.05）和较重的婴儿（P<0.002）与显著更大的脐血体积相关，而母亲的年龄、产次和婴儿性别没有显著影响。
发现唯一显著影响TNC和CD34+细胞计数的因素是婴儿和胎盘的重量以及收集的脐带血体积。由于这些因素具有预后意义，对它们的分析将有助于决定哪些脐血单位应进行处理并冷冻保存用于脐血库储存及后续移植。
