InScientiaFides Foundation, San Marino, Republic of San Marino.
Department of Experimental Medicine, Sapienza University, Rome, Italy.
Stem Cell Res Ther. 2022 Jun 11;13(1):246. doi: 10.1186/s13287-022-02915-y.
Cord blood plays a very important role in stem cell transplantation and therapy with an emerging implication also in regenerative medicine. The number of cells available in a single cord blood unit (CBU), in particular, the CD34+ and total nucleated cell (TNC) content influences the transplantation clinical outcome. We analysed a very large series of CBUs, collected for private banking without any specific volume restriction, to deeply investigate the best predictors of cord blood stem cells content.
Maternal and neonatal clinic laboratory data of a total 2583 UCBs were obtained from the InScientiaFides cord blood bank based in Republic of San Marino. Univariate and multivariate analysis were conducted to better interpret the data and to build a predictive model to select, the CBU with high CD34+ content.
Our univariate analysis shows that seasonality and the geographical area affects the quality of umbilical cord blood. Gestational age, babie's gender and birth weight have a positive correlation with CB TNC content. The babie's birth weight affects positively also CD34+ content and CBU volume while the cesarean delivery affect the CB volume only. Our predictive model, based on multivariate analysis, shows that male babie's, gestational age lower to 39 weeks, cesarean delivery and CBUs with a content of TNC higher than 3.44 × 10 (group A) have a significant higher CD34+ content than group B (female babie's, gestational age higher than 39 weeks and vaginal delivery). The group A have a 37.5% of CBUs with a concentration of CD34+ > 2 × 10, while no CBUs with high concentration of CD34+ were detect in group B.
This study, conducted on a very large series of CBUs without any specific volume constraint, highlighted the prenatal and maternal factors that significantly influence the quality of the CBU collected. Specifically, it highlights that volume is not the best predictor of CD34+ CBU content; for this reason it cannot be taken into consideration alone for the analysis of the collected samples. Our final aim is to identify relevant factors, immediately available, that help to choice UCB with high CD34+ cell content, especially in simultaneous deliveries.
脐带血在干细胞移植和治疗中起着非常重要的作用，在再生医学中也有新的应用。单个脐带血单位（CBU）中可用细胞的数量，特别是 CD34+和总核细胞（TNC）的含量，会影响移植的临床结果。我们分析了大量的 CBU，这些 CBU 是为私人银行收集的，没有任何特定的体积限制，以深入研究脐带血干细胞含量的最佳预测因子。
从位于圣马力诺共和国的 InScientiaFides 脐带血库获得了总共 2583 个 UCB 的母体和新生儿临床实验室数据。进行了单变量和多变量分析，以更好地解释数据，并建立一个预测模型来选择 CD34+含量高的 CBU。
我们的单变量分析表明，季节性和地理位置会影响脐带血的质量。胎龄、婴儿的性别和出生体重与 CBU 的 TNC 含量呈正相关。婴儿的出生体重也会对 CD34+含量和 CBU 体积产生积极影响，而剖宫产只影响 CBU 体积。我们的多变量分析预测模型显示，男婴、胎龄低于 39 周、剖宫产和 TNC 含量高于 3.44×10 的 CBU（A 组）比 B 组（胎龄高于 39 周和阴道分娩的女婴）具有更高的 CD34+含量。A 组中有 37.5%的 CBU 的 CD34+浓度>2×10，而 B 组中没有检测到高浓度的 CD34+。
这项在没有任何特定体积限制的大量 CBU 上进行的研究强调了产前和母体因素对采集的 CBU 质量有显著影响。具体来说，它强调了体积不是 CD34+CBU 含量的最佳预测因子；因此，不能单独考虑体积对采集样本的分析。我们的最终目标是确定相关因素，这些因素可以帮助选择 CD34+细胞含量高的 UCB，特别是在同时分娩的情况下。
