Singh Prabhakar, Rizvi Syed Ibrahim
Department of Biochemistry, University of Allahabad, Allahabad 211002, India.
Int J Cell Biol. 2015;2015:630246. doi: 10.1155/2015/630246. Epub 2015 Sep 2.
Curcumin ((1E,6E)-1,7-Bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione), the yellow biphenolic pigment isolated from turmeric (Curcuma longa), has various medicinal benefits through antioxidation, anti-inflammation, cardiovascular protection, immunomodulation, enhancing of the apoptotic process, and antiangiogenic property. We explored the effects of curcumin in vitro (10(-5) M to 10(-8) M) and in vivo (340 and 170 mg/kg b.w., oral) on Na(+)/K(+) ATPase (NKA), Na(+)/H(+) exchanger (NHE) activity, and membrane lipid hydroperoxides (ROOH) in control and experimental oxidative stress erythrocytes of Wistar rats. As a result, we found that curcumin potently modulated the membrane transporters activity with protecting membrane lipids against hydro-peroxidation in control as well as oxidatively challenged erythrocytes evidenced by stimulation of NKA, downregulation of NHE, and reduction of ROOH in the membrane. The observed results corroborate membrane transporters activity with susceptibility of erythrocyte membrane towards oxidative damage. Results explain the protective mechanism of curcumin against oxidative stress mediated impairment in ions-transporters activity and health beneficial effects.
姜黄素（(1E,6E)-1,7-双(4-羟基-3-甲氧基苯基)-1,6-庚二烯-3,5-二酮）是从姜黄（Curcuma longa）中分离出的黄色双酚类色素，通过抗氧化、抗炎、心血管保护、免疫调节、促进凋亡过程和抗血管生成特性等具有多种药用益处。我们研究了姜黄素在体外（10(-5) M至10(-8) M）和体内（340和170 mg/kg体重，口服）对Wistar大鼠对照和实验性氧化应激红细胞中Na(+)/K(+) ATP酶（NKA）、Na(+)/H(+)交换体（NHE）活性以及膜脂质氢过氧化物（ROOH）的影响。结果发现，姜黄素能有效调节膜转运蛋白活性，保护膜脂质免受氢过氧化作用，在对照以及氧化应激的红细胞中均有体现，表现为刺激NKA、下调NHE以及降低膜中的ROOH。观察结果证实了膜转运蛋白活性与红细胞膜对氧化损伤的易感性之间的关系。结果解释了姜黄素对氧化应激介导的离子转运蛋白活性损伤的保护机制以及有益健康的作用。
