从伊朗医院分离出的产超广谱β-内酰胺酶（ESBL）和不产ESBL的大肠埃希菌中的磺胺耐药基因（sul）M
Sulfonamide Resistance Genes (sul) M in Extended Spectrum Beta Lactamase (ESBL) and Non-ESBL Producing Escherichia coli Isolated From Iranian Hospitals.
作者信息
Arabi Hadis, Pakzad Iraj, Nasrollahi Ayat, Hosainzadegan Hasan, Azizi Jalilian Farid, Taherikalani Morovvat, Samadi Naser, Monadi Sefidan Allireza
机构信息
Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, IR Iran.
Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran ; Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, IR Iran.
出版信息
Jundishapur J Microbiol. 2015 Jul 25;8(7):e19961. doi: 10.5812/jjm.19961v2. eCollection 2015 Jul.
BACKGROUND
Extensive use of cotrimoxazole has been associated with increasing level of Escherichia coli resistance.
OBJECTIVES
In the current study, we focused on assessing the prevalence of E. coli resistance to cotrimoxazole and frequency of its associated genes.
MATERIALS AND METHODS
One-hundred and forty-four E. coli isolates were identified during March 2007 to April 2012 at Ilam hospitals and Milad (Tehran) hospital. Antibiotic susceptibility for screening of resistance isolates was done by the Kirby-Bauer method. The sul1, sul2, sul3, dfrA1, dfrA5, int1, blaTEM, blaSHV and CTX-M genes were detected by polymerase chain reaction (PCR) amplification. Plasmid curing was done for identifying correlations between resistance genes and plasmids.
RESULTS
Amongst the 144 E. coli isolates, seventy-two (50%) Extended Spectrum Beta Lactamase (ESBL)-producing and seventy-two (50%) non-ESBL-producing E. coli isolates were identified; eighty-seven isolates (60.41%) were resistant to cotrimoxazole. Frequencies of sul1, sul2 and sul3, were 81% (116 isolates), 67% (96 isolates) and 2.29% (three isolates), respectively. Furthermore, 50.57% (72 isolates) had sul1 and sul2, 2.29% (3 isolates) contained sul2 and sul3, and 2.29% (three isolates) contained sul1, sul2 and sul3 genes, simultaneously. Thirty-four (39.1%) of the isolates had the dfrA1 gene. Five (5.7%) of the isolates had the dfrA5 gene. Sixty-eight (78.2%) strains contained the int1 gene. Furthermore, dfrA1 and dfrA5 were present in three (3.4%) of the isolates. The results showed that of the ESBL-producing isolates, 85.2% (n = 122), 53.2% (n = 76) and 26.1% (n = 37) were blaTEM, blaSHV and CTX-M harboring isolates, respectively.
CONCLUSIONS
Our study indicated a high frequency of cotrimoxazole resistance gene in E. coli isolates from Ilam and Tehran (Milad) hospitals, and sul genes had a major role in cotrimoxazole resistance of these isolates.
背景
广泛使用复方新诺明与大肠杆菌耐药性水平的上升有关。
目的
在本研究中，我们着重评估大肠杆菌对复方新诺明的耐药率及其相关基因的频率。
材料与方法
2007年3月至2012年4月期间，在伊拉姆医院和德黑兰米拉德医院鉴定出144株大肠杆菌分离株。采用 Kirby-Bauer 法进行抗生素敏感性检测以筛选耐药菌株。通过聚合酶链反应（PCR）扩增检测sul1、sul2、sul3、dfrA1、dfrA5、int1、blaTEM、blaSHV和CTX-M基因。进行质粒消除以确定耐药基因与质粒之间的相关性。
结果
在144株大肠杆菌分离株中，鉴定出72株（50%）产超广谱β-内酰胺酶（ESBL）的大肠杆菌分离株和72株（50%）非产ESBL的大肠杆菌分离株；87株分离株（60.41%）对复方新诺明耐药。sul1、sul2和sul3的频率分别为81%（116株分离株）、67%（96株分离株）和2.29%（3株分离株）。此外，50.57%（72株分离株）同时具有sul1和sul2，2.29%（3株分离株）同时含有sul2和sul3，2.29%（3株分离株）同时含有sul1、sul2和sul3基因。34株（39.1%）分离株具有dfrA1基因。5株（5.7%）分离株具有dfrA5基因。68株（78.2%）菌株含有int1基因。此外，3株（3.4%）分离株同时存在dfrA1和dfrA5。结果显示，在产ESBL的分离株中，分别有85.2%（n = 122）、53.2%（n = 76）和26.1%（n = 37）的菌株携带blaTEM、blaSHV和CTX-M基因。
结论
我们的研究表明，来自伊拉姆和德黑兰（米拉德）医院的大肠杆菌分离株中复方新诺明耐药基因的频率较高，且sul基因在这些分离株对复方新诺明的耐药性中起主要作用。
Zotero 插件