热量限制与福尔马林诱导的炎症：大鼠模型的实验研究

Caloric Restriction and Formalin-Induced Inflammation: An Experimental Study in Rat Model.

作者信息

Nozad Aisan, Safari Mir Bahram, Saboory Ehsan, Derafshpoor Leila, Mohseni Moghaddam Parvaneh, Ghaffari Farzaneh, Naseri Mohsen

机构信息

Traditional Iranian Medicine Department, Medicine Faculty, Shahed University, Tehran, IR Iran.

Faculty of Medicine, Urmia University of Medical Sciences, Urmia, IR Iran.

出版信息

Iran Red Crescent Med J. 2015 Jul 23;17(7):e22590. doi: 10.5812/ircmj.22590v2. eCollection 2015 Jul.

DOI:10.5812/ircmj.22590v2

PMID:26421173

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4584080/

Abstract

BACKGROUND

Acute and chronic inflammations are difficult to control. Using chemical anti-inflammatory medications along with their complications considerably limit their use. According to Traditional Iranian Medicine (TIM), there is an important relation between inflammation and Imtila (food and blood accumulation in the body); food reduction or its more modern equivalent Caloric Restriction (CR) may act against both Imtila and inflammation.

OBJECTIVES

This experimental study aimed to investigate the effect of 30% reduction in daily calorie intake on inflammation in rats.

MATERIALS AND METHODS

A total of 18 male rats (Rattus rattus) weighing 220 to 270 g were obtained. Then, the inflammation was induced by injecting formalin in their paws. Next, the rats were randomized by generating random numbers into two equal groups (9 + 9) putting on either normal diet (controls) or a similar diet with 30% reduction of calorie (cases). Paw volume changes were recorded twice per day by one observer in both groups using a standard plethysmometer for 8 consecutive days. Serum C-reactive protein (CRP), Erythrocyte Sedimentation Rate (ESR), complete blood count (erythrocyte, platelet, and white blood cell) and hemoglobin were compared between the groups.

RESULTS

Decline of both body weight and paw volume was significantly more prominent in the case than in the control rats within the study period (P < 0.001 and < 0.001, respectively). Paw volume decrease was more prominent after day 3. On day 8, serum CRP-positive (1 or 2 +) rats were more frequent in ad libitum fed group comparing with those received CR (33.3% vs. 11.1%). This difference, however, was insignificant (P = 0.58). At the same time, mean ESR was significantly higher in the control rats comparing with that in the case group (29.00 ± 2.89 h vs. 14.00 ± 1.55 h; P = 0.001). Other serum parameters were not significantly different between the two groups at endpoint.

CONCLUSIONS

Rats fed with a 30% calorie-restricted diet in comparison with to ad libitum fed controls for 8 days had significantly more prominent regression of inflammation.

摘要

背景

急慢性炎症难以控制。使用化学抗炎药物及其并发症极大地限制了它们的应用。根据传统伊朗医学（TIM），炎症与体内食物和血液积聚（Imtila）之间存在重要关联；减少食物摄入或其更现代的等效方式热量限制（CR）可能对Imtila和炎症都有作用。

目的

本实验研究旨在调查每日热量摄入减少30%对大鼠炎症的影响。

材料与方法

总共获得18只体重220至270克的雄性大鼠（褐家鼠）。然后，通过在它们的爪子中注射福尔马林诱导炎症。接下来，通过生成随机数将大鼠随机分为两个相等的组（9 + 9），一组给予正常饮食（对照组），另一组给予热量减少30%的类似饮食（实验组）。两组均由一名观察者使用标准体积描记器每天记录两次爪子体积变化，持续8天。比较两组之间的血清C反应蛋白（CRP）、红细胞沉降率（ESR）、全血细胞计数（红细胞、血小板和白细胞）以及血红蛋白。

结果

在研究期间，实验组大鼠的体重和爪子体积下降均明显比对照组更显著（分别为P < 0.001和< 0.001）。第3天后爪子体积减小更为明显。在第8天，自由进食组血清CRP呈阳性（1或2 +）的大鼠比接受热量限制的组更频繁（33.3%对11.1%）。然而，这种差异不显著（P = 0.58）。同时，对照组大鼠的平均ESR明显高于实验组（29.00 ± 2.89小时对14.00 ± 1.55小时；P = 0.001）。在实验终点时，两组之间的其他血清参数没有显著差异。