定量PCR与常规PCR检测水和沉积物样本中的人类腺病毒
QUANTITATIVE VS. CONVENTIONAL PCR FOR DETECTION OF HUMAN ADENOVIRUSES IN WATER AND SEDIMENT SAMPLES.
作者信息
Staggemeier Rodrigo, Bortoluzzi Marina, Heck Tatiana Moraes da Silva, Spilki Fernando Rosado, Almeida Sabrina Esteves de Matos
机构信息
Universidade Feevale, Novo Hamburgo, RS, BR.
出版信息
Rev Inst Med Trop Sao Paulo. 2015 Jul-Aug;57(4):299-303. doi: 10.1590/S0036-46652015000400005.
Abstract
Human Adenoviruses (HAdV) are notably resistant in the environment. These agents may serve as effective indicators of fecal contamination, and may act as causative agents of a number of different diseases in human beings. Conventional polymerase chain reaction (PCR) and, more recently, quantitative PCR (qPCR) are widely used for detection of viral agents in environmental matrices. In the present study PCR and SYBR(r)Green qPCR assays were compared for detection of HAdV in water (55) and sediments (20) samples of spring and artesian wells, ponds and streams, collected from dairy farms. By the quantitative methodology HAdV were detected in 87.3% of the water samples and 80% of the sediments, while by the conventional PCR 47.3% and 35% were detected in water samples and sediments, respectively.
摘要
人腺病毒（HAdV）在环境中具有显著的抗性。这些病原体可作为粪便污染的有效指示物，并可能成为人类多种不同疾病的病原体。传统的聚合酶链反应（PCR）以及最近的定量PCR（qPCR）被广泛用于检测环境基质中的病毒病原体。在本研究中，比较了PCR和SYBR(r) Green qPCR检测方法，用于检测从奶牛场采集的泉水井、自流井、池塘和溪流的水（55份）和沉积物（20份）样本中的HAdV。通过定量方法，在87.3%的水样和80%的沉积物中检测到HAdV，而通过传统PCR分别在47.3%的水样和35%的沉积物中检测到HAdV。
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