巴西伯南布哥州的腹泻暴发，与豚鼠气单胞菌产生的一种耐热细胞毒素肠毒素有关。

DIARRHEA OUTBREAK IN PERNAMBUCO, BRAZIL, ASSOCIATED WITH A HEAT-STABLE CYTOTOXIC ENTEROTOXIN PRODUCED BY Aeromonas caviae.

作者信息

Lopes Ana Carolina Amaral, Martins Luciano Moura, Gatti Maria Silvia Viccari, Falavina Dos Reis Cristhiane Moura, Hofer Ernesto, Yano Tomomasa

机构信息

Evolution and Bioagents Department, State University of Campinas, Campinas, SP, BR.

Enteric and Special Pathogens Infectious Diseases Department, Instituto Adolfo Lutz, Sao Paulo, SP, BR.

出版信息

Rev Inst Med Trop Sao Paulo. 2015 Jul-Aug;57(4):349-51. doi: 10.1590/S0036-46652015000400013.

DOI:10.1590/S0036-46652015000400013

PMID:26422161

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4616922/

Abstract

In the present study enterotoxic and cytotoxic activities of twenty Aeromonas caviae strains were examined. They originated from fecal specimens of patients with acute diarrhea during an outbreak in Brazil in 2004. Culture supernatants of fourteen strains (70%) caused fluid accumulation in rabbit ileal intestinal loops and in suckling mice assays, and also showed a cytotoxic activity in Vero and Caco-2 cells. The enterotoxic and cytotoxic factors were heat-stable after culture supernatants treatment at 100 ºC. The results revealed that A. caviae strains produce a putative diarrheagenic virulence factor, a heat-stable cytotoxic enterotoxin that could be linked to the diarrhea outbreak that took place in Brazil.

摘要

在本研究中，检测了20株豚鼠气单胞菌的肠毒素和细胞毒素活性。它们源自2004年巴西一次疫情期间急性腹泻患者的粪便标本。14株菌株（70%）的培养上清液在兔回肠肠袢和乳鼠试验中引起液体蓄积，并且在Vero细胞和Caco-2细胞中也表现出细胞毒素活性。培养上清液在100℃处理后，肠毒素和细胞毒素因子具有热稳定性。结果显示，豚鼠气单胞菌菌株产生一种假定的致腹泻毒力因子，即一种热稳定的细胞毒素性肠毒素，这可能与巴西发生的腹泻疫情有关。