Xie Hongtu, Li Jianwei, Zhang Bin, Wang Lianfeng, Wang Jingkuan, He Hongbo, Zhang Xudong
State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110164, China.
Department of Agriculture and Environmental Sciences, Tennessee State University, Nashville, TN 37209, USA.
Sci Rep. 2015 Oct 1;5:14687. doi: 10.1038/srep14687.
Glomalin-related soil protein (GRSP) contributes to the formation and maintenance of soil aggregates, it is however remains unclear whether long-term intensive manure amendments alter soil aggregates stability and whether GRSP regulates these changes. Based on a three-decade long fertilization experiment in northeast China, this study examined the impact of long-term manure input on soil organic carbon (SOC), total and easily extractable GRSP (GRSPt and GRSPe) and their respective allocations in four soil aggregates (>2000 μm; 2000-250 μm; 250-53 μm; and <53 μm). The treatments include no fertilization (CK), low and high manure amendment (M1, M2), chemical nitrogen, phosphorus and potassium fertilizers (NPK), and combined manure and chemical fertilizers (NPKM1, NPKM2). Though SOC, GRSPe and GRSPt in soil and SOC in each aggregate generally increased with increasing manure input, GRSPt and GRSPe in each aggregate showed varying changes with manure input. Both GRSP in macroaggregates (2000-250 μm) were significantly higher under low manure input, a pattern consistent with changes in soil aggregate stability. Constituting 38~49% of soil mass, macroaggregates likely contributed to the nonlinear changes of aggregate stability under manure amendments. The regulatory process of GRSP allocations in soil aggregates has important implications for manure management under intensive agriculture.
球囊霉素相关土壤蛋白（GRSP）有助于土壤团聚体的形成和维持，然而，长期大量施用有机肥是否会改变土壤团聚体稳定性以及GRSP是否调节这些变化仍不清楚。基于中国东北地区长达三十年的施肥试验，本研究考察了长期施入有机肥对土壤有机碳（SOC）、总球囊霉素相关土壤蛋白和易提取球囊霉素相关土壤蛋白（GRSPt和GRSPe）及其在四种土壤团聚体（>2000μm；2000 - 250μm；250 - 53μm；<53μm）中各自分配的影响。处理包括不施肥（CK）、低量和高量有机肥施用（M1、M2）、化学氮磷钾肥（NPK）以及有机肥与化肥配施（NPKM1、NPKM2）。尽管土壤中的SOC、GRSPe和GRSPt以及各团聚体中的SOC通常随有机肥施用量增加而增加，但各团聚体中的GRSPt和GRSPe随有机肥施用量呈现不同变化。低量有机肥投入下大团聚体（2000 - 250μm）中的GRSP均显著更高，这一模式与土壤团聚体稳定性变化一致。大团聚体占土壤质量的38% - 49%，可能导致了有机肥施用下团聚体稳定性的非线性变化。GRSP在土壤团聚体中的分配调控过程对集约化农业下的有机肥管理具有重要意义。
