Carvalho Maria Reggiani Azevedo, dos Santos da Silva Marcos André, de Sousa Brito Carla Adriana Rodrigues, Campelo Viriato, Kuga Milton Carlos, Tonetto Mateus Rodrigues, De Jesus Tavarez Rudys Rodolfo, Bandéca Matheus Coelho, Pinzan-Vercelino Celia Regina Maio
Department of Postgraduation in Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil.
Department of Parasitology and Microbiology, Federal University of Piaui, Teresina, PI, Brazil.
J Contemp Dent Pract. 2015 Aug 1;16(8):619-23. doi: 10.5005/jp-journals-10024-1731.
To compare the antimicrobial activity of the chemical substances--70% isopropyl alcohol, 2% glutaraldehyde (GTA) and 0.25% peracetic acid (PAA) in disinfecting orthodontic pliers contaminated in vitro with Streptococcui mutani, Staphylococci aureui and Candida albicani.
Distal end cutter pliers were divided into five groups: group 1 (negative control--sterilized pliers), group 2 (positive control--sterilized plier, subsequently contaminated), group 3 (disinfected with 70% isopropyl alcohol, friction method), group 4 (disinfected with 2% GTA, immersion method for 30 minutes), group 5 (disinfected with 0.25% peracetic acid (PAA), immersion method for 10 minutes). After the pliers were treated with one disinfectant and submitted to microbiological evaluation (by counting colony forming units), they were submitted to the same cleansing, sterilizing and contaminating processes, and were used in the following groups (crossover and washout study). The two-factor analysis of variance (ANOVA) test, followed by the Tukey test, was used to compare the groups.
The results showed that there was no statistically significant difference between the three tested disinfectants.
Although there were no statistically significant differences between the disinfectants, the chemical agents 2% glutaraldehyde and 0.25% PAA were effective in inhibiting the growth of the three microorganisms tested; however, 70% isopropyl alcohol was unable to completely eliminate S. aureui.
The chemical substances 2% glutaraldehyde and 0.25% PAA completely eliminated the microorganisms tested.
比较化学物质——70%异丙醇、2%戊二醛（GTA）和0.25%过氧乙酸（PAA）对在体外被变形链球菌、金黄色葡萄球菌和白色念珠菌污染的正畸钳的抗菌活性。
远端切割钳分为五组：第1组（阴性对照——已灭菌的钳子），第2组（阳性对照——已灭菌的钳子，随后被污染），第3组（用70%异丙醇消毒，摩擦法），第4组（用2%戊二醛消毒，浸泡30分钟），第5组（用0.25%过氧乙酸（PAA）消毒，浸泡10分钟）。钳子用一种消毒剂处理并进行微生物学评估（通过计算菌落形成单位）后，再进行相同的清洁、灭菌和污染过程，并用于后续组（交叉和洗脱研究）。采用双因素方差分析（ANOVA）检验，随后进行Tukey检验来比较各组。
结果表明，三种受试消毒剂之间无统计学显著差异。
虽然消毒剂之间无统计学显著差异，但化学试剂2%戊二醛和0.25%过氧乙酸能有效抑制三种受试微生物的生长；然而，70%异丙醇无法完全消除金黄色葡萄球菌。
化学物质2%戊二醛和0.25%过氧乙酸能完全消除受试微生物。
