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正畸钳的有效消毒

Effective disinfection of orthodontic pliers.

作者信息

Wichelhaus Andrea, Bader Friedel, Sander Franz Günter, Krieger Dorothea, Mertens Thomas

机构信息

Clinic of Orthodontics and Pedodontics, University of Basle, Basle, Switzerland.

出版信息

J Orofac Orthop. 2006 Sep;67(5):316-36. doi: 10.1007/s00056-006-0622-9.

Abstract

OBJECTIVE

Pathogenic microbes may be transmitted directly from the orthodontist to the patient or from the patient to the doctor, and indirectly from patient to patient. The latter may occur via contaminated instruments or surfaces, and is referred to as cross-contamination. The objective of this study was to evaluate the extent of bacterial contamination of orthodontic pliers and the efficacy of the disinfection techniques applied after clinical use. We also sought to examine under standardized conditions the virucidal, bactericidal and fungicidal effects of disinfection techniques used in practice.

MATERIALS AND METHODS

The efficacy of various disinfection methods was determined after clinical use in-vivo on 10 test subjects and in-vitro with deliberate contamination. The following disinfection methods were tested: 1. Iso-Septol spray 2. Incidur spray 3. Trough disinfection in combination with 5% Sekusept Plus solution 4. Ultrasound bath in combination with 5% Sekusept Plus solution 5. Thermal disinfection For in-vitro contamination we used the test organisms Staphylococcus aureus, Escherichia coli, Candida albicans, Coxsackie virus B4, HSV 1, and Adenovirus type 5. The tests were carried out six to eight times for each organism. The Weingart pliers and distalend cutters were tested. The criteria for effective disinfection were a reduction in infectiosity of five log steps (for bacteria and fungi) or four log steps (viruses). Statistical analysis was carried out using the Wilcoxon and Whitney U-test.

RESULTS

The presence of contamination following clinical use was not adequately eliminated with all disinfection methods. The spray methods exhibited shortcomings in disinfection. For the type of contamination defined, trough disinfection with 5% Sekusept Plus and the Incidur and Iso-Septol spray disinfection methods provided insufficient disinfection. Conversely, the ultrasound bath with 5% Sekusept Plus solution and steam disinfection met the criteria for effective disinfection for all microbes. No statistically significant difference was found between the oiled and unoiled states. In some cases, there were slightly higher rates of contamination with the Weingart pliers as with the distalend cutters. However, these were not statistically significant.

CONCLUSIONS

It should be possible to disinfect lipophilic viruses and the usual bacterial infections adequately with all methods, provided that the use of sprays and trough disinfection is preceded by cleaning with brush and water, followed by drying. With hydrophilic viruses, however, the spray and trough disinfection methods are limited in their efficacy and cannot be considered adequate. Exclusively chemical methods are therefore less effective than thermal or physical-chemical methods. Thermal disinfection and the ultrasound bath in combination with 5% Sekusept Plus are clearly superior to spray disinfection and trough disinfection alone. The ultrasound bath and thermal disinfection can therefore be recommended for the disinfection of orthodontic pliers. We recommend that the pliers be cleaned beforehand due to their uneven surfaces.

摘要

目的

致病微生物可能直接从正畸医生传播给患者,或从患者传播给医生,也可能间接在患者之间传播。后者可能通过受污染的器械或表面发生,被称为交叉污染。本研究的目的是评估正畸钳的细菌污染程度以及临床使用后所应用消毒技术的效果。我们还试图在标准化条件下检验实际使用的消毒技术的杀病毒、杀菌和杀真菌效果。

材料与方法

在10名受试对象身上进行临床体内使用以及在体外进行故意污染后,测定各种消毒方法的效果。测试了以下消毒方法:1. Iso - Septol喷雾 2. Incidur喷雾 3. 与5% Sekusept Plus溶液联合的槽式消毒 4. 与5% Sekusept Plus溶液联合的超声浴 5. 热消毒。对于体外污染,我们使用了测试微生物金黄色葡萄球菌、大肠杆菌、白色念珠菌、柯萨奇病毒B4、单纯疱疹病毒1型和5型腺病毒。每种微生物的测试进行了六至八次。对Weingart钳和远端切割器进行了测试。有效消毒的标准是感染性降低五个对数级(对于细菌和真菌)或四个对数级(对于病毒)。使用Wilcoxon和Whitney U检验进行统计分析。

结果

并非所有消毒方法都能充分消除临床使用后的污染。喷雾方法在消毒方面存在不足。对于所定义的污染类型,用5% Sekusept Plus进行的槽式消毒以及Incidur和Iso - Septol喷雾消毒方法消毒不充分。相反,5% Sekusept Plus溶液的超声浴和蒸汽消毒对所有微生物均符合有效消毒标准。在涂油和未涂油状态之间未发现统计学上的显著差异。在某些情况下,Weingart钳的污染率略高于远端切割器。然而,这些差异无统计学意义。

结论

只要在使用喷雾和槽式消毒之前先用刷子和水清洁,然后干燥,所有方法应该都能够充分消毒亲脂性病毒和常见的细菌感染。然而,对于亲水性病毒,喷雾和槽式消毒方法的效果有限,不能认为是充分的。因此,单纯化学方法不如热或物理化学方法有效。热消毒以及5% Sekusept Plus溶液联合的超声浴明显优于单独的喷雾消毒和槽式消毒。因此,超声浴和热消毒可推荐用于正畸钳的消毒。由于钳子表面不平整,我们建议预先进行清洁。

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