三种实时荧光定量PCR检测方法用于检测年轻孕妇沙眼衣原体和淋病奈瑟菌的比较
Comparison of three real-time PCR assays for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in young pregnant women.
作者信息
Peuchant Olivia, de Diego Sabrina, Le Roy Chloé, Frantz-Blancpain Sandrine, Hocké Claude, Bébéar Cécile, de Barbeyrac Bertille
机构信息
Univ. Bordeaux, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France; INRA, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France; Centre Hospitalier Universitaire de Bordeaux, Laboratoire de Bactériologie, Bordeaux, France.
Univ. Bordeaux, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France; INRA, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France.
出版信息
Diagn Microbiol Infect Dis. 2015 Dec;83(4):335-7. doi: 10.1016/j.diagmicrobio.2015.09.002. Epub 2015 Sep 7.
We compared 3 commercial real-time PCR assays, the Abbott RealTime CT/NG, the cobas® 4800 CT/NG, and the Cepheid Xpert® CT/NG, for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in vaginal swabs collected prospectively from pregnant women aged <25 years. The overall agreement among 2 assays ranged from 98.9% to 99.5% with a kappa score between 0.94 and 0.97 for C. trachomatis. For N. gonorrhoeae, the overall agreement was 100%. All kits allowed prompt and specific results for C. trachomatis and N. gonorrhoeae in young pregnant women.
我们比较了3种商业实时荧光定量PCR检测方法，即雅培实时CT/NG检测法、cobas® 4800 CT/NG检测法和赛沛Xpert® CT/NG检测法，用于检测前瞻性收集的年龄小于25岁孕妇阴道拭子中的沙眼衣原体和淋病奈瑟菌。两种检测方法之间的总体一致性在98.9%至99.5%之间，沙眼衣原体的kappa值在0.94至0.97之间。对于淋病奈瑟菌，总体一致性为100%。所有试剂盒都能为年轻孕妇的沙眼衣原体和淋病奈瑟菌提供快速且特异的检测结果。
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