Poullet Nausicaa, Braendle Christian
Institut de Biologie Valrose, CNRS UMR7277, Parc Valrose, Nice, Cedex 02, 06108, France.
INSERM U1091, Nice, Cedex 02, 06108, France.
Methods Mol Biol. 2015;1327:221-9. doi: 10.1007/978-1-4939-2842-2_16.
Wild populations of the model organism C. elegans allow characterization of natural genetic variation underlying diverse phenotypic traits. Here we provide a simple protocol on how to sample and rapidly identify C. elegans wild isolates. We outline how to find suitable habitats and organic substrates, followed by describing isolation and identification of C. elegans live cultures based on easily recognizable morphological characteristics, molecular barcodes and/or mating tests. This protocol uses standard laboratory equipment and requires no prior knowledge of C. elegans biology.
模式生物秀丽隐杆线虫的野生种群有助于对多种表型性状背后的自然遗传变异进行表征。在此，我们提供了一个关于如何采样并快速鉴定秀丽隐杆线虫野生分离株的简单方案。我们概述了如何找到合适的栖息地和有机基质，接着描述了基于易于识别的形态特征、分子条形码和/或交配试验来分离和鉴定秀丽隐杆线虫活培养物的方法。该方案使用标准实验室设备，且无需具备秀丽隐杆线虫生物学的先验知识。
