Université Côte d'Azur, CNRS, Inserm, IBV, Nice, France.
URZ, INRAE, Petit-Bourg (Guadeloupe), France.
Methods Mol Biol. 2022;2468:283-292. doi: 10.1007/978-1-0716-2181-3_15.
Wild populations of the model organism C. elegans represent a valuable resource, allowing for genetic characterization underlying natural phenotypic variation. Here we provide a simple protocol on how to sample and rapidly identify C. elegans wild isolates. We outline how to find suitable habitats and organic substrates, followed by describing isolation and identification of C. elegans live cultures based on easily recognizable morphological characteristics, molecular barcodes, and mating tests. This protocol uses standard laboratory equipment and requires little prior knowledge of C. elegans biology.
模式生物秀丽隐杆线虫的野生种群是一种宝贵的资源，可用于对自然表型变异的遗传特征进行分析。本文提供了一种简单的方案，用于采集和快速鉴定秀丽隐杆线虫的野生分离株。我们概述了如何寻找合适的栖息地和有机基质，然后描述了根据易于识别的形态特征、分子条码和交配试验分离和鉴定秀丽隐杆线虫活体培养物的方法。本方案使用标准的实验室设备，并且几乎不需要对秀丽隐杆线虫生物学有预先的了解。
