Research Center for Analytical Sciences, Northeastern University , Box 332, Shenyang, Liaoning 110819, China.
Institute of Molecular Medicine, College of Life and Health Sciences, Northeastern University , Shenyang, Liaoning 110819, China.
Anal Chem. 2015 Nov 3;87(21):10902-9. doi: 10.1021/acs.analchem.5b02453. Epub 2015 Oct 12.
It is of great importance to develop novel and sensitive sensing materials for the detection of proteins and microorganisms to fulfill the demand of disease diagnosis. As the selectivity and sensitivity of sensing systems are highly dependent on the receptor, the fluorescent sensor array with imidazolium ionic liquids (ILs) and ionic liquid-quantum dots conjugates as semiselective receptors is developed for protein/bacteria differential sensing or discrimination. The IL sensing system formed by 1,3-dibutylimidazolium chloride (BBimCl), 1,3-diethylimidazolium bromine (EEimBr), 1,3-dibutylimidazolium bromine (BBimBr), 1,3-dihexylimidazolium bromine (HHimBr), and 1,3-dioctylimidazolium bromine (OOimBr) and the IL@QDs/QDs sensing system formed by CdTe, BBimCl@CdTe, EEimBr@CdTe, BBimBr@CdTe, and HHimBr@CdTe are tested, by transferring the interaction binding difference between receptors and proteins to the fluorescent response pattern. The IL sensing system is applied to the identification of 48 samples (8 proteins at 500 nM) with an accuracy of 91.7%. For the IL@QDs/QDs sensing system, 8 proteins are completely distinguished with 100% accuracy at a very low concentration level of 10 nM. Remarkably, 36 training cases (6 strains of bacteria from 3 different species) are discriminated with 100% (OD600 of 0.1).
开发用于检测蛋白质和微生物的新型和灵敏感测材料对于满足疾病诊断的需求非常重要。由于感测系统的选择性和灵敏度高度依赖于受体，因此开发了具有离子液体（ILs）和离子液体-量子点缀合物作为半选择性受体的荧光传感器阵列，用于蛋白质/细菌的差分感测或鉴别。由 1,3-二丁基咪唑氯（BBimCl）、1,3-二乙基咪唑溴（EEimBr）、1,3-二丁基咪唑溴（BBimBr）、1,3-二己基咪唑溴（HHimBr）和 1,3-二辛基咪唑溴（OOimBr）组成的 IL 感测系统以及由 CdTe、BBimCl@CdTe、EEimBr@CdTe、BBimBr@CdTe 和 HHimBr@CdTe 组成的 IL@QDs/QDs 感测系统进行了测试，通过将受体与蛋白质之间的相互作用差异转化为荧光响应模式。IL 感测系统用于识别 48 个样本（500 nM 时 8 种蛋白质），准确率为 91.7%。对于 IL@QDs/QDs 感测系统，在非常低的浓度水平（10 nM）下，8 种蛋白质的识别准确率为 100%。值得注意的是，36 个训练案例（来自 3 个不同物种的 6 株细菌）的区分准确率为 100%（OD600 为 0.1）。
