Gnessi L, Isidori A, Bolotti M, Altamura S, Ulisse S, Jannini E A, Fabbri A, Spera G
Department of Andrology, Policlinico Umberto I, University of Rome La Sapienza, Italy.
Endocrinology. 1989 Jan;124(1):558-60. doi: 10.1210/endo-124-1-558.
Purified adult rat Leydig cells were found to produce gastrin-releasing peptide (GRP) by radioimmunoassay (RIA). Gel chromatography of the extracted material showed a single peak of GRP immunoreactivity. Further high pressure liquid chromatography (HPLC) analysis resolved the extract into two peaks that closely resembled the C-terminal fragment of GRP, GRP18-27 and GRP14-27. Immunohistochemical studies revealed specific staining for GRP in the Leydig cells of adult rat testis. These results demonstrate, by a number of independent criteria, that rat Leydig cells contain substances which behave like authentic GRP-like peptides. Since the peptides appear to be of local origin, a paracrine function within the rat testis is suggested.
通过放射免疫测定法（RIA）发现，纯化的成年大鼠睾丸间质细胞能产生胃泌素释放肽（GRP）。对提取物进行凝胶色谱分析，结果显示GRP免疫反应性呈单峰。进一步的高压液相色谱（HPLC）分析将提取物分离为两个峰，这两个峰与GRP的C端片段GRP18 - 27和GRP14 - 27极为相似。免疫组织化学研究显示，成年大鼠睾丸间质细胞中GRP呈特异性染色。这些结果通过多个独立标准证明，大鼠睾丸间质细胞含有与真正的GRP样肽表现相似的物质。由于这些肽似乎起源于局部，因此提示其在大鼠睾丸中具有旁分泌功能。
