Chailyan Anna, Marcatili Paolo
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark.
Methods Mol Biol. 2015;1348:205-14. doi: 10.1007/978-1-4939-2999-3_18.
The role of proteins as very effective immunogens for the generation of antibodies is indisputable. Nevertheless, cases in which protein usage for antibody production is not feasible or convenient compelled the creation of a powerful alternative consisting of synthetic peptides. Synthetic peptides can be modified to obtain desired properties or conformation, tagged for purification, isotopically labeled for protein quantitation or conjugated to immunogens for antibody production. The antibodies that bind to these peptides represent an invaluable tool for biological research and discovery. To better understand the underlying mechanisms of antibody-antigen interaction here we present a pipeline developed by us to structurally classify immunoglobulin antigen binding sites and to infer key sequence residues and other variables that have a prominent role in each structural class.
蛋白质作为产生抗体的非常有效的免疫原，其作用是无可争议的。然而，在某些情况下，使用蛋白质来生产抗体不可行或不方便，这促使人们创造了一种强大的替代物，即合成肽。合成肽可以进行修饰以获得所需的特性或构象，添加标签用于纯化，进行同位素标记用于蛋白质定量，或与免疫原偶联用于抗体生产。与这些肽结合的抗体是生物学研究和发现的宝贵工具。为了更好地理解抗体 - 抗原相互作用的潜在机制，我们在此展示我们开发的一个流程，用于对免疫球蛋白抗原结合位点进行结构分类，并推断在每个结构类别中起重要作用的关键序列残基和其他变量。
