Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark.
Methods Mol Biol. 2024;2821:195-204. doi: 10.1007/978-1-0716-3914-6_15.
The role of proteins as very effective immunogens for the generation of antibodies is indisputable. Nevertheless, cases in which protein usage for antibody production is not feasible or convenient compelled the creation of a powerful alternative consisting of synthetic peptides. Synthetic peptides can be modified to obtain desired properties or conformation, tagged for purification, isotopically labeled for protein quantitation or conjugated to immunogens for antibody production. The antibodies that bind to these peptides represent an invaluable tool for biological research and discovery. To better understand the underlying mechanisms of antibody-antigen interaction, here, we present a pipeline developed by us to structurally classify immunoglobulin antigen binding sites and to infer key sequence residues and other variables that have a prominent role in each structural class.
蛋白质作为非常有效的免疫原，用于产生抗体是无可争议的。然而，在某些情况下，使用蛋白质来生产抗体是不可行或不方便的，这就迫使人们创造了一种强大的替代品，即合成肽。合成肽可以进行修饰，以获得所需的特性或构象，标记用于纯化，同位素标记用于蛋白质定量，或与免疫原结合用于生产抗体。与这些肽结合的抗体是生物研究和发现的宝贵工具。为了更好地理解抗体-抗原相互作用的潜在机制，在这里，我们展示了一个由我们开发的用于结构分类免疫球蛋白抗原结合位点的流程，并推断出在每个结构类别中起主要作用的关键序列残基和其他变量。
