Kong Ling-Bo, Ma Qun, Gao Jie, Qiu Guo-Song, Wang Li-Xia, Zhao Shu-Min, Bao Yong-Gang, Liu Qing-Quan
Department of Emergency Medicine, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, 100700, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100029, China.
Chin J Integr Med. 2015 Dec;21(12):916-21. doi: 10.1007/s11655-015-2089-2. Epub 2015 Sep 30.
To investigate the effect of Qiguiyin Decoction, QGYD) on multidrug-resistant Pseudomonas aeruginosa infection in Sprague-Dawley (SD) rats.
A pseudomonal infection model in SD rats was established by injecting multidrug-resistant P. aeruginosa intraperitoneally. Infected rats were randomized into four groups treated with Pure water, QGYD, ceftazidime, or combined QGYD and ceftazidime. Blood samples were obtained from the abdominal aorta. Serum was then collected and analyzed by peptide array for immune responsiveness to multidrug-resistant beta-lactamase proteins, including Verona integronen-coded metallo-beta-lactamase 1 (VIM-1), Sao Paulo metallo-beta-lactamase 1 (SPM-1), and Temoniera (TEMs). Blood levels of interleukin-1β (IL-1β), interleukin-4 (IL-4), and interferon-γ (IFN-γ) were assessed by enzyme-linked immunosorbent assay.
QGYD enhanced antibody reactivity against VIM-1 [epitopes 7-11 and 36-40] and TEM-1 [epitopes 26-27, 52-55, and 66-70]. QGYD treatment restored the compromised antibody reactivity against VIM-1 [epitopes 53-54 and 56-58] and SPM-1 [epitopes 16-19 and 82-85] following pseudomonal infection. Serum levels of IL-1β and Th1/Th2 in the rats were significantly elevated following pseudomonal infection (P<0.05 orP<0.01). In contrast, QGYD and combination QGYD and ceftazidime treatment restored the elevated serum IL-1β and Th1/Th2 levels to normal (P>0.05).
QGYD improves the immune response to pseudomonal infection in rats by stimulating the production of protective antibodies against drug-resistant proteins VIM-1, SPM-1, and TEM-1. In addition, it protects the immune system and maintains immune responsiveness by restoring IL-1β and Th1/Th2 levels.
探讨芪归银汤（QGYD）对斯普拉格-道利（SD）大鼠多重耐药铜绿假单胞菌感染的影响。
通过腹腔注射多重耐药铜绿假单胞菌建立SD大鼠假单胞菌感染模型。将感染大鼠随机分为四组，分别用纯水、芪归银汤、头孢他啶或芪归银汤与头孢他啶联合治疗。从腹主动脉采集血样。然后收集血清，通过肽阵列分析对多重耐药β-内酰胺酶蛋白的免疫反应性，包括维罗纳整合子编码的金属β-内酰胺酶1（VIM-1）、圣保罗金属β-内酰胺酶1（SPM-1）和替莫内拉（TEMs）。通过酶联免疫吸附测定法评估白细胞介素-1β（IL-1β）、白细胞介素-4（IL-4）和干扰素-γ（IFN-γ）的血药浓度。
芪归银汤增强了针对VIM-1[表位7-11和36-40]和TEM-1[表位26-27、52-55和66-70]的抗体反应性。芪归银汤治疗恢复了假单胞菌感染后针对VIM-1[表位53-54和56-58]和SPM-1[表位16-19和82-85]受损的抗体反应性。假单胞菌感染后大鼠血清IL-1β水平和Th1/Th2显著升高（P<0.05或P<0.01）。相比之下，芪归银汤以及芪归银汤与头孢他啶联合治疗将升高的血清IL-1β水平和Th1/Th2恢复至正常（P>0.05）。
芪归银汤通过刺激产生针对耐药蛋白VIM-1、SPM-1和TEM-1的保护性抗体，改善大鼠对假单胞菌感染的免疫反应。此外，它通过恢复IL-1β水平和Th1/Th2来保护免疫系统并维持免疫反应性。
