Cui Xuran, Xu Xiaolong, Hu Yahui, Li Rui, Liu Qingquan
Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, 100010, People's Republic of China.
Beijing Institute of Chinese Medicine, Beijing, 100010, People's Republic of China.
Infect Drug Resist. 2023 May 18;16:3073-3084. doi: 10.2147/IDR.S405033. eCollection 2023.
Qiguiyin decoction (QGYD) was a traditional Chinese medicine (TCM) used to treat infection in China. This study investigated the therapeutic effect and the potential mechanism of QGYD on carbapenem-resistant (CRPA) infection.
Pulmonary infections were induced in mice by CRPA. The therapeutic effect of QGYD was evaluated by lung index and pulmonary pathology. The potential effects of QGYD on intestinal flora were detected by gut microbiome. The overall metabolism regulation of QGYD in blood was investigated by metabonomics. Next, the correlation between intestinal flora and metabolites was analyzed to illustrate the relationship between the regulatory effects of QGYD on metabolites and the beneficial effects of intestinal flora.
QGYD has significant therapeutic effect on CRPA infection. QGYD profoundly inhibited the excessive accumulation of and at phylum and genus levels, respectively. Eleven potential metabolites that were abnormally expressed by CRPA infection and significantly reversed by QGYD were identified. Ten of the eleven metabolites significantly regulated by QGYD were related to showed significant positive correlation with DL-lactic acid, phenylalanine and other metabolites and significant negative correlation with vitamin k1. At the genus level, was closely related to metabolites significantly regulated by QGYD. was positively correlated with metabolites such as Dl-lactate and negatively correlated with vitamin k1.
QGYD can improve CRPA infection and has the effect of regulating intestinal flora and metabolism. It was a promising drug against infection.
芪归银汤（QGYD）是中国用于治疗感染的一种中药。本研究探讨了芪归银汤对耐碳青霉烯类肺炎克雷伯菌（CRPA）感染的治疗效果及潜在机制。
用CRPA诱导小鼠发生肺部感染。通过肺指数和肺病理学评估芪归银汤的治疗效果。通过肠道微生物组检测芪归银汤对肠道菌群的潜在影响。采用代谢组学研究芪归银汤在血液中的整体代谢调控。接下来，分析肠道菌群与代谢产物之间的相关性，以阐明芪归银汤对代谢产物的调节作用与肠道菌群有益作用之间的关系。
芪归银汤对CRPA感染有显著治疗效果。芪归银汤分别在门和属水平上显著抑制了[具体菌属]的过度积累。鉴定出11种因CRPA感染异常表达且被芪归银汤显著逆转的潜在代谢产物。芪归银汤显著调节的11种代谢产物中有10种与[具体代谢途径或相关物质]有关，与DL-乳酸、苯丙氨酸等代谢产物呈显著正相关，与维生素K1呈显著负相关。在属水平上，[具体菌属]与芪归银汤显著调节的代谢产物密切相关。[具体菌属]与Dl-乳酸等代谢产物呈正相关，与维生素K1呈负相关。
芪归银汤可改善CRPA感染，具有调节肠道菌群和代谢的作用。它是一种有前景的抗感染药物。
