Mechanism of Qiguiyin Decoction Treats Pulmonary Infection Caused by Based on Gut Microbiota and Metabolomics.

BACKGROUND

Qiguiyin decoction (QGYD) was a traditional Chinese medicine (TCM) used to treat infection in China. This study investigated the therapeutic effect and the potential mechanism of QGYD on carbapenem-resistant (CRPA) infection.

MATERIALS AND METHODS

Pulmonary infections were induced in mice by CRPA. The therapeutic effect of QGYD was evaluated by lung index and pulmonary pathology. The potential effects of QGYD on intestinal flora were detected by gut microbiome. The overall metabolism regulation of QGYD in blood was investigated by metabonomics. Next, the correlation between intestinal flora and metabolites was analyzed to illustrate the relationship between the regulatory effects of QGYD on metabolites and the beneficial effects of intestinal flora.

RESULTS

QGYD has significant therapeutic effect on CRPA infection. QGYD profoundly inhibited the excessive accumulation of and at phylum and genus levels, respectively. Eleven potential metabolites that were abnormally expressed by CRPA infection and significantly reversed by QGYD were identified. Ten of the eleven metabolites significantly regulated by QGYD were related to showed significant positive correlation with DL-lactic acid, phenylalanine and other metabolites and significant negative correlation with vitamin k1. At the genus level, was closely related to metabolites significantly regulated by QGYD. was positively correlated with metabolites such as Dl-lactate and negatively correlated with vitamin k1.

CONCLUSION

QGYD can improve CRPA infection and has the effect of regulating intestinal flora and metabolism. It was a promising drug against infection.