Fallahabadi Zahra Rafiei, Noori Daloii Mohammad Reza, Mahdian Reza, Behjati Farhkondeh, Shokrgozar Mohamad Ali, Abolhasani Maryam, Asgari Mojgan, Shahrokh Hossein
Molecular Medicine Department, Pasteur Institute of Iran, Tehran, Iran; National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran; Medical Genetics Department, Tehran University of Medical Sciences, Tehran, Iran.
Medical Genetics Department, Tehran University of Medical Sciences, Tehran, Iran.
Gene. 2016 Jan 10;575(2 Pt 3):755-60. doi: 10.1016/j.gene.2015.09.068. Epub 2015 Sep 28.
Phosphatase and tensin homolog (PTEN) gene aberration and trans membrane protease, serine 2 (TMPRSS2)-v-ets avian erythroblastosis virus E26 oncogene homolog (ERG) fusion are the most prevalent genomic events in prostate cancer. In this study we aimed to evaluate the frequency of PTEN alteration and TMPRSS2-ERG fusion and possible link between these two biomarkers in Iranian men.
We assessed 42 fresh frozen tissue samples of prostate cancer (PCA) obtained by radical prostatectomy, interrogating the TMPRSS2-ERG fusion gene along with PTEN gene status using Real Time PCR and FISH methods.
Using Real Time PCR we identified the TMPRSS2-ERG fusion in 64% (27/42) of tumor samples, which was confirmed by FISH technique, giving 21 positive samples with deletion, suggesting the presence of TMPRSS2-ERG fusion gene. By contrast, PTEN deletion was detected in 52% (11/21) of PCA samples, which all showed low expression in Real Time. Concomitance of PTEN deletion or low expression and TMPRSS2-ERG fusion was present in PCA samples (P=0.005). All of the PTEN deletion samples showed TMPRSS2-ERG fusion, (11/11, 100%) while not all of the TMPRSS2-ERG fusion positive samples showed PTEN deletion. None of 29 cases of BPH and 8 cases of normal zone of tumor tissue showed TMPRSS2-ERG fusion.
These results indicate that PTEN loss occurs in cooperation with TMPRSS2-ERG fusion in PCA. While the majority of PCA samples harbor TMPRSS2-ERG fusion as well as PTEN gene deletion, normal tissues do not show these molecular aberrations.
磷酸酶和张力蛋白同源物（PTEN）基因畸变以及跨膜蛋白酶丝氨酸2（TMPRSS2）-v-ets禽成红细胞增多症病毒E26癌基因同源物（ERG）融合是前列腺癌中最常见的基因组事件。在本研究中，我们旨在评估伊朗男性中PTEN改变和TMPRSS2-ERG融合的频率以及这两种生物标志物之间的可能联系。
我们评估了42例通过根治性前列腺切除术获得的前列腺癌（PCA）新鲜冷冻组织样本，使用实时PCR和FISH方法检测TMPRSS2-ERG融合基因以及PTEN基因状态。
使用实时PCR，我们在64%（27/42）的肿瘤样本中鉴定出TMPRSS2-ERG融合，FISH技术证实了这一结果，有21个阳性样本存在缺失，提示存在TMPRSS2-ERG融合基因。相比之下，在52%（11/21）的PCA样本中检测到PTEN缺失，所有这些样本在实时检测中均显示低表达。PCA样本中存在PTEN缺失或低表达与TMPRSS2-ERG融合的共存情况（P=0.005）。所有PTEN缺失样本均显示TMPRSS2-ERG融合（11/11，100%），而并非所有TMPRSS2-ERG融合阳性样本都显示PTEN缺失。29例良性前列腺增生（BPH）病例和8例肿瘤组织正常区域病例均未显示TMPRSS2-ERG融合。
这些结果表明，在PCA中PTEN缺失与TMPRSS2-ERG融合共同发生。虽然大多数PCA样本同时存在TMPRSS2-ERG融合以及PTEN基因缺失，但正常组织未显示这些分子畸变。
