Zur Gila, Klement Eyal
Veterinary Teaching Hospital (Zur), Koret School of Veterinary Medicine (Zur, Klement), Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel.
Can J Vet Res. 2015 Oct;79(4):316-22.
Morphological studies comparing normal and diseased ear canals use primarily subjective scoring. The aim of this study was to compare normal and severely affected ears in dogs with objective measurements using ImageJ software. Ear canals were harvested from cadavers with normal ears and from dogs that underwent total ear canal ablation for unresolved otitis. Histopathology samples from ear canals were evaluated by semi-quantitative scoring and also by using ImageJ-software for histomorphometric measurements. The normal ears were compared to the severely affected ears using the 2 methods. The 2 methods were significantly (P < 0.0001) correlated for epidermal hyperplasia, ceruminous gland dilation, and hyperplasia and tissue inflammation, which were significantly greater in the severely affected ears (P < 0.0001). This study demonstrated that there is a very high correlation between the 2 methods for the most markedly affected components of otitis externa and that ImageJ software can be efficiently used to measure and evaluate ear canal histomorphometry.
比较正常和患病耳道的形态学研究主要采用主观评分。本研究的目的是使用ImageJ软件通过客观测量来比较正常犬和患有严重耳道疾病的犬的耳朵。从具有正常耳朵的尸体以及因未治愈的中耳炎而接受全耳道切除的犬身上获取耳道。通过半定量评分以及使用ImageJ软件进行组织形态计量学测量来评估耳道的组织病理学样本。使用这两种方法将正常耳朵与严重患病耳朵进行比较。对于表皮增生、耵聍腺扩张、增生和组织炎症，这两种方法具有显著相关性（P < 0.0001），而这些在严重患病耳朵中显著更严重（P < 0.0001）。本研究表明，对于外耳道最明显受影响的成分，这两种方法之间存在非常高的相关性，并且ImageJ软件可有效地用于测量和评估耳道组织形态计量学。
