Bonyadi Mohammad Hossein Jabbarpoor, Bonyadi Morteza, Ahmadieh Hamid, Fotuhi Nikoo, Shoeibi Nasser, Saadat Saeed, Yagubi Zakieh
Department of Ophthalmology, Gonabad University of Medical Sciences, Gonabad, Iran ; Center of Excellence for Biodiversity, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Center of Excellence for Biodiversity, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
J Ophthalmic Vis Res. 2015 Apr-Jun;10(2):155-9. doi: 10.4103/2008-322X.163781.
To investigate tumor necrosis factor (TNF)-α gene polymorphisms in advanced dry-type age-related macular degeneration (AMD) in a population from Northeastern Iran.
In this case-control study, 50 patients with geographic macular atrophy and 73 gender-matched controls were enrolled. Genomic deoxyribonucleic acid (DNA) was extracted from the peripheral blood. Polymerase chain reaction was performed to analyze 2 candidate single nucleotide polymorphisms in the TNF-α gene, namely -1031 thymine (T)/cytosine (C) and -308 guanine (G)/adenine (A).
The distribution of the - 1031 T/C genotype was TT, 62%; TC, 36%; CC, 2% in the patients and TT, 60%; TC, 36%; CC, 4% in the controls (P = 0.94). Genotype analysis of TNF-α -308 also revealed no significant difference in distribution between patients (G, 78%; GA, 22%; AA, 0%) and controls (GG, 74%; GA, 23%; AA, 3%) (P = 0.51). None of the haplotypes nor alleles of studied TNF-α polymorphisms were significantly associated with advanced dry-type AMD.
The findings of this study show that polymorphisms in the TNF-α gene, do not play an important role in dry-type AMD in the studied population.
在伊朗东北部人群中研究晚期干性年龄相关性黄斑变性（AMD）患者的肿瘤坏死因子（TNF）-α基因多态性。
在这项病例对照研究中，纳入了50例地图状黄斑萎缩患者和73例性别匹配的对照者。从外周血中提取基因组脱氧核糖核酸（DNA）。采用聚合酶链反应分析TNF-α基因中的2个候选单核苷酸多态性，即-1031位胸腺嘧啶（T）/胞嘧啶（C）和-308位鸟嘌呤（G）/腺嘌呤（A）。
患者中-1031 T/C基因型的分布为TT，62%；TC，36%；CC，2%；对照者中TT，60%；TC，36%；CC，4%（P = 0.94）。TNF-α -308的基因型分析也显示，患者（G，78%；GA，22%；AA，0%）和对照者（GG，74%；GA，23%；AA，3%）之间的分布无显著差异（P = 0.51）。所研究的TNF-α多态性的单倍型和等位基因均与晚期干性AMD无显著关联。
本研究结果表明，TNF-α基因多态性在该研究人群的干性AMD中不发挥重要作用。
