Anand Akshay, Sharma Neel K, Singh Ramandeep, Gupta Amod, Prabhakar Sudesh, Jindal Neeru, Bhatt Arvind K, Sharma Suresh K, Gupta Pawan K
1] Neuroscience Researh Lab, Department of Neurology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India [2].
1] Department of Ophthalmology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India [2].
Sci Rep. 2014 Feb 18;4:4114. doi: 10.1038/srep04114.
It has been postulated that there is a link between age related degenerative diseases and cancer. The TNF-related apoptosis-inducing ligand (TRAIL) has been shown to selectively kill tumor cells by binding to pro-apoptotic and anti-apoptotic receptors. Our aim was to study the levels of anti-apoptotic receptor (DcR1) in age related macular degeneration (AMD) and controls. AMD patients (115) were classified into two groups: Dry and Wet AMD. Wet AMDs were further classified into occult, predominant classic and minimal classic. 61 healthy individuals were recruited as normal controls. After normalization with total protein, DcR1 levels were analyzed by ELISA. Mann Whitney U-statistic was used for analysis of DcR1 ELISA results. We have observed DcR1 levels in serum sample which were significantly lower in AMD patients as compared to controls (p = 0.001). On the other hand, we did not find difference in DcR1 levels between wet and dry AMD. The present study defines the plausible role of DcR1 in AMD pathology signifying a new therapeutic target for AMD.
据推测，与年龄相关的退行性疾病和癌症之间存在联系。肿瘤坏死因子相关凋亡诱导配体（TRAIL）已被证明可通过与促凋亡和抗凋亡受体结合来选择性杀死肿瘤细胞。我们的目的是研究抗凋亡受体（DcR1）在年龄相关性黄斑变性（AMD）患者及对照组中的水平。115例AMD患者被分为两组：干性AMD和湿性AMD。湿性AMD进一步分为隐匿性、典型性为主和极小典型性。招募61名健康个体作为正常对照。用总蛋白进行标准化后，通过酶联免疫吸附测定（ELISA）分析DcR1水平。采用曼-惠特尼U检验分析DcR1 ELISA结果。我们观察到，与对照组相比，AMD患者血清样本中的DcR1水平显著降低（p = 0.001）。另一方面，我们未发现湿性和干性AMD患者的DcR1水平存在差异。本研究确定了DcR1在AMD病理中的可能作用，这意味着AMD有了一个新的治疗靶点。
