Ichihara T, Nakao A, Sakamoto J, Nonami T, Harada A, Watanabe T, Takagi H, Nagura H
Department of Surgery, Nagoya University School of Medicine, Japan.
J Surg Oncol. 1989 Jan;40(1):8-16. doi: 10.1002/jso.2930400105.
Intrapancreatic extension of pancreatic duct cell carcinoma in 19 resectable cases was evaluated by both hematoxylin and eosin (H&E) staining and quick immunostaining using antibodies against cancer-associated antigens--e.g., CA19-9, DUPAN 2, and CEA--on the intraoperative frozen-section biopsy during the course of laparotomy. By H&E staining, malignant lesions were identified in four of 19 cases (21%). By the immunostaining method, however, cancer cells were detected in 10 of 19 cases (52.6%). This immunostaining method was particularly useful in defining cancer cells surrounded by dense fibrous connective tissue, in which cancer cells are usually difficult to evaluate by H&E staining. Thus, the application of such immunostaining, together with H&E staining, on the cryostat sections of biopsy specimens may provide important information for the appropriate operative method. Retrospective study of permanent sections revealed that pathological diagnosis by quick immunostaining was more accurate than diagnosis by H&E staining.
通过苏木精和伊红（H&E）染色以及使用针对癌症相关抗原（如CA19-9、DUPAN 2和CEA）的抗体在剖腹手术过程中对术中冰冻切片活检进行快速免疫染色，对19例可切除的胰腺导管细胞癌的胰腺内扩展情况进行了评估。通过H&E染色，在19例中有4例（21%）发现了恶性病变。然而，通过免疫染色方法，在19例中有10例（52.6%）检测到癌细胞。这种免疫染色方法在确定被致密纤维结缔组织包围的癌细胞方面特别有用，在这种情况下，癌细胞通常很难通过H&E染色进行评估。因此，在活检标本的低温切片上应用这种免疫染色以及H&E染色，可能为选择合适的手术方法提供重要信息。对永久切片的回顾性研究表明，快速免疫染色的病理诊断比H&E染色的诊断更准确。
