Ichihara T, Nakao A, Suzuki Y, Sakamoto J, Nonami T, Harada A, Nagura H, Takagi H
Department of Surgery II, Nagoya University School of Medicine, Japan.
J Surg Oncol. 1989 Nov;42(3):209-14. doi: 10.1002/jso.2930420316.
The rapid immunostaining of gastrointestinal cancer-associated antigens, CA19-9, CEA, DUPAN2, and CA50, for intraoperative pathological diagnosis of pancreatic cancer can be completed in only 13 min by applying microwave irradiation, which was used to accelerate the incubation of the primary antibody. Only 3 s irradiation at 500 W for fresh-frozen sections produced specific antigen staining of greater intensity than that obtained with longer incubation, and tissue preservation was satisfactory with minimal non-specific background staining. This rapid immunoperoxidase staining method on the cryostat section of pancreatic biopsy specimens can provide important information for determining an appropriate operative method for pancreatic cancer patients.
通过应用微波辐射加速一抗孵育，仅需13分钟即可完成胃肠道癌相关抗原CA19-9、癌胚抗原（CEA）、DUPAN2和CA50的快速免疫染色，用于胰腺癌的术中病理诊断。对于新鲜冷冻切片，在500瓦功率下照射仅3秒，产生的特异性抗原染色强度高于长时间孵育所获得的强度，并且组织保存良好，非特异性背景染色极少。这种对胰腺活检标本冷冻切片的快速免疫过氧化物酶染色方法可为确定胰腺癌患者合适的手术方法提供重要信息。
