用于发光成像和生物正交光催化的钌共轭物在细胞内蛋白质标记中的应用
In cellulo protein labelling with Ru-conjugate for luminescence imaging and bioorthogonal photocatalysis.
作者信息
Sadhu Kalyan K, Lindberg E, Winssinger N
机构信息
University of Geneva, School of Chemistry and Biochemsitry, NCCR Chemical Biology, 30 quai Ernest Ansermet, Geneva, Switzerland.
出版信息
Chem Commun (Camb). 2015 Dec 4;51(93):16664-6. doi: 10.1039/c5cc05405b. Epub 2015 Oct 1.
PMID:26426098
Abstract
Labelling of proteins with a luminescent ruthenium complex enables the direct visualization and photocatalytic reduction of aryl azide in live cells. The confinement of catalysis to the labeled proteins was visualized using an azide-based immolative linker releasing a precipitating dye.
摘要
用发光钌配合物标记蛋白质能够在活细胞中直接可视化芳基叠氮化物并进行光催化还原。使用释放沉淀染料的基于叠氮化物的牺牲性连接体，将催化作用限制在标记蛋白质上的情况得以可视化。
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