Moses K, Pepple D, Singh P
Department of Basic Medical Sciences, The University of the West Indies, Kingston 7, Jamaica, West Indies.
Pharmacology Section, Department of Basic Medical Sciences, The University of the West Indies, Kingston 7, Jamaica, West Indies.
West Indian Med J. 2015 Jun;64(3):186-8. doi: 10.7727/wimj.2014.067. Epub 2015 May 18.
Epigallocatechin-3-gallate (EGCG) is a major ingredient present in green tea, which has a high antioxidant activity. In this study, the effect of EGCG was investigated on paraquat-induced haemolysis of erythrocyte membrane. Erythrocytes were incubated in 0.03, 0.3, 3.0 and 30 mg/mL EGCG, respectively and exposed to 30 mg/mL of paraquat for 10 minutes. The effect of paraquat was determined by an analysis of the osmotic fragility of the erythrocytes. The results showed that EGCG (30 mg/mL) significantly (p < 0.05) reduced the haemolysis of erythrocytes exposed to paraquat (5.0 mg/mL). This suggests that EGCG may have a protective effect on paraquat-induced erythrocyte membrane haemolysis and that consumption of green tea, with high EGCG concentration, could ameliorate the deleterious effect of paraquat toxicity on the haemolysis of erythrocyte membrane.
表没食子儿茶素-3-没食子酸酯（EGCG）是绿茶中的主要成分，具有很高的抗氧化活性。在本研究中，研究了EGCG对百草枯诱导的红细胞膜溶血的影响。将红细胞分别置于0.03、0.3、3.0和30mg/mL的EGCG中孵育，并暴露于30mg/mL的百草枯中10分钟。通过分析红细胞的渗透脆性来确定百草枯的作用。结果表明，EGCG（30mg/mL）显著（p<0.05）降低了暴露于百草枯（5.0mg/mL）的红细胞的溶血。这表明EGCG可能对百草枯诱导的红细胞膜溶血具有保护作用，并且饮用高EGCG浓度的绿茶可以改善百草枯毒性对红细胞膜溶血的有害影响。
