Department of Biochemistry, Islamiah College, Vaniyambadi, 635752 Tamil Nadu, India.
Pharmacol Rep. 2010 Sep-Oct;62(5):891-9. doi: 10.1016/s1734-1140(10)70349-2.
The protective effect of epigallocatechin-3-gallate (EGCG) against cigarette smoke (CS) induced alterations in human erythrocyte was studied using an in vitro model. Hemolysis, carboxyhemoglobin, osmotic fragility, hemin, lipid peroxidation (LPO), protein thiol, protein carbonyl, glutathione, antioxidant enzymes, membrane bound ATPases and erythrocyte ghost protein were assessed to investigate the effect of EGCG. Erythrocytes were incubated with CS and/or 10 μM EGCG under physiological conditions of temperature and pH for 2 h. CS significantly increased the percentage of hemolysis, carboxyhemoglobin, hemin, LPO and osmotic fragility in human erythrocytes whereas EGCG pretreatment significantly reduced all the above parameters. The levels of protein carbonyls significantly increased whereas the level of protein thiol decreased significantly in erythrocytes incubated with CS. EGCG pretreatment significantly decreased the levels of carbonyls and increased the level of protein thiol. The level of glutathione, antioxidant enzyme and membrane bound ATPases were decreased significantly in erythrocytes incubated with CS. However, EGCG pretreatment significantly increased the activities of GSH, antioxidant enzymes and membrane bound ATPases. CS incubated erythrocytes showed a progressive loss of the cytoskeleton proteins and formation of low molecular weight bands and protein aggregates. EGCG pretreatment of CS incubated erythrocytes showed a near normal protein profile compared to that of control erythrocytes. The present study divulges that EGCG can reduce the abnormalities of cigarette smoking by ameliorating the oxidative stress. This finding raises the possibility that EGCG may provide protection from CS induced toxicity.
采用体外模型研究了表没食子儿茶素没食子酸酯（EGCG）对香烟烟雾（CS）诱导的人红细胞改变的保护作用。通过测定溶血、碳氧血红蛋白、渗透脆性、高铁血红素、脂质过氧化（LPO）、蛋白质巯基、蛋白质羰基、谷胱甘肽、抗氧化酶、膜结合 ATP 酶和红细胞血影蛋白来研究 EGCG 的作用。将红细胞在生理温度和 pH 条件下与 CS 和/或 10 μM EGCG 孵育 2 小时。CS 显著增加了人红细胞的溶血率、碳氧血红蛋白、高铁血红素、LPO 和渗透脆性，而 EGCG 预处理显著降低了所有上述参数。与 CS 孵育的红细胞中蛋白质羰基水平显著升高，而蛋白质巯基水平显著降低。EGCG 预处理可显著降低羰基水平并增加蛋白质巯基水平。与 CS 孵育的红细胞中谷胱甘肽、抗氧化酶和膜结合 ATP 酶的水平显著降低。然而，EGCG 预处理显著增加了 GSH、抗氧化酶和膜结合 ATP 酶的活性。CS 孵育的红细胞显示细胞骨架蛋白逐渐丢失，并形成低分子量带和蛋白质聚集体。与 CS 孵育的红细胞相比，EGCG 预处理的红细胞的蛋白质谱接近正常。本研究表明，EGCG 通过改善氧化应激可以减少吸烟引起的异常。这一发现表明，EGCG 可能提供对 CS 诱导的毒性的保护。
