Paletzki Ronald, Gerfen Charles R
Laboratory of Systems Neuroscience, NIMH, Bethesda, Maryland.
Curr Protoc Neurosci. 2015 Oct 1;73:1.25.1-1.25.21. doi: 10.1002/0471142301.ns0125s73.
Whole-brain reconstruction of the mouse enables comprehensive analysis of the distribution of neurochemical markers, the distribution of anterogradely labeled axonal projections or retrogradely labeled neurons projecting to a specific brain site, or the distribution of neurons displaying activity-related markers in behavioral paradigms. This unit describes a method to produce whole-brain reconstruction image sets from coronal brain sections with up to four fluorescent markers using the freely available image-processing program FIJI (ImageJ).
小鼠全脑重建能够全面分析神经化学标记物的分布、顺行标记的轴突投射或逆行标记的投射到特定脑区的神经元的分布，或者在行为范式中显示活动相关标记物的神经元的分布。本单元介绍了一种使用免费的图像处理程序FIJI（ImageJ）从冠状脑切片生成具有多达四种荧光标记物的全脑重建图像集的方法。
