Lv Xiaopei, Fu Kaiqiang, Li Weishi, Wang Yu, Wang Jifang, Li Huatao, Tian Wenru, Cao Rongfeng
Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.
Can J Physiol Pharmacol. 2015 Nov;93(11):967-71. doi: 10.1139/cjpp-2015-0003. Epub 2015 May 6.
Endometritis is one of the main diseases that harms the dairy cow industry. Tanshinone IIA (TIIA), a fat-soluble alkaloid isolated from Salviae miltiorrhizae, has been reported to have potent anti-inflammatory properties. However, the anti-inflammatory effects of TIIA on a mouse model of lipopolysaccharide (LPS)-induced endometritis remain to be elucidated. The purpose of the present study was to investigate the effects of TIIA on LPS-induced mouse endometritis. TIIA was intraperitoneally injected 1 h before and 12 h after perfusion of LPS into the uterus. A histological examination was then performed, and the concentrations of myeloperoxidase (MPO) and nitric oxide (NO) in the uterine tissue were determined. The levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in a homogenate of the uterus were detected by enzyme-linked immunosorbent assay. The extent of phosphorylation of IκBα and p65 was detected by Western blotting. TIIA markedly reduced the infiltration of neutrophils, suppressed MPO activity and the concentration of NO, and attenuated the expression of TNF-α and IL-1β. Furthermore, TIIA inhibited the phosphorylation of the nuclear factor-kappa B (NF-κB) p65 subunit and the degradation of its inhibitor IκBα. All the results suggest that TIIA has strong anti-inflammatory effects on LPS-induced mouse endometritis.
子宫内膜炎是危害奶牛养殖业的主要疾病之一。丹参酮IIA（TIIA）是从丹参中分离出的一种脂溶性生物碱，据报道具有强大的抗炎特性。然而，TIIA对脂多糖（LPS）诱导的小鼠子宫内膜炎模型的抗炎作用仍有待阐明。本研究的目的是探讨TIIA对LPS诱导的小鼠子宫内膜炎的影响。在向子宫灌注LPS前1小时和灌注后12小时腹腔注射TIIA。然后进行组织学检查，并测定子宫组织中髓过氧化物酶（MPO）和一氧化氮（NO）的浓度。通过酶联免疫吸附测定法检测子宫匀浆中肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的水平。通过蛋白质印迹法检测IκBα和p65的磷酸化程度。TIIA显著减少了中性粒细胞的浸润，抑制了MPO活性和NO浓度，并减弱了TNF-α和IL-1β的表达。此外，TIIA抑制了核因子-κB（NF-κB）p65亚基的磷酸化及其抑制剂IκBα的降解。所有结果表明，TIIA对LPS诱导的小鼠子宫内膜炎具有强大的抗炎作用。
