Branco Mário S D, Sousa Denise M C, Sousa Daíse M C, Monteiro Joelma D, Costa Diego M P, Almeida Junior Renato F, Lima Tábata L C, Farias Kleber J S, Medeiros Ralfo C, Queiroz Maria Goretti L, Gil Laura H V G, Fernandes José V, Nogueira Rita M R, Araújo Josélio M G
Laboratory of Molecular Biology for Infectious Diseases and Cancer, Federal University of Rio Grande do Norte, Natal, Brazil.
Laboratory of Flavivirus, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.
Trop Med Int Health. 2015 Dec;20(12):1707-10. doi: 10.1111/tmi.12612. Epub 2015 Oct 19.
To present results of virological surveillance and epidemiological aspects of dengue in the State of Rio Grande do Norte, Brazil.
A total of 1581 cases, reported from 2010 to 2012 at various health centres in the state, were analysed by viral isolation and/or RT-PCR for viral detection and typing. To identify whether different genotypes were circulating in the state during this period, sequencing of the complete E gene for DENV (1485 bp in length) was performed directly from patient serum samples.
All four serotypes of dengue virus circulated in Rio Grande do Norte, with the introduction of DENV-4 in the state in 2011. In 2012, DENV-4 represented 100% of positive confirmed cases. 53.97% of cases occurred in Natal. Case numbers peaked in April (21%) and May (23%). Genetic characterisation of circulating strains confirmed the circulation of genotypes V, south-east Asian/American and II, respectively, for DENV-1, DENV-2 and DENV-4.
This work furthers a better understanding of dengue viruses in the State of Rio Grande do Norte. Strengthening control efforts in the region is important considering the impact of dengue.
呈现巴西北里奥格兰德州登革热的病毒学监测结果及流行病学情况。
对该州各卫生中心2010年至2012年报告的共1581例病例进行病毒分离和/或逆转录聚合酶链反应分析，以进行病毒检测和分型。为确定在此期间该州是否有不同基因型在传播，直接从患者血清样本中对登革病毒完整E基因（长度为1485 bp）进行测序。
登革病毒的所有四种血清型都在北里奥格兰德州传播，2011年该州引入了登革病毒4型。2012年，登革病毒4型占确诊阳性病例的100%。53.97%的病例发生在纳塔尔。病例数在4月（21%）和5月（23%）达到峰值。对传播毒株的基因特征分析证实，登革病毒1型、2型和4型分别有基因型V、东南亚/美洲型和II型在传播。
这项工作有助于更好地了解北里奥格兰德州的登革病毒。考虑到登革热的影响，加强该地区的防控工作很重要。
