Merl-Pham Juliane, Gruhn Fabian, Hauck Stefanie M
Research Unit Protein Science, Helmholtz Zentrum München, German Research Center for Environmental Health GmbH, Ingolstaedter Landstr.1, 85764, Munich, Germany.
Adv Exp Med Biol. 2016;854:785-91. doi: 10.1007/978-3-319-17121-0_105.
Age-related macular degeneration (AMD) is a medical condition usually affecting older adults and resulting in a loss of vision in the macula, the center of the visual field. The dry form of this disease presents with atrophy of the retinal pigment epithelium, resulting in the detachment of the retina and loss of photoreceptors. Cigarette smoke is one main risk factor for dry AMD and increases the risk of developing the disease by three times. In order to understand the influence of cigarette smoke on retinal pigment epithelial cells, cultured human ARPE-19 cells were treated with cigarette smoke extract for 24 h. Using quantitative mass spectrometry more than 3000 proteins were identified and their respective abundances were compared between cigarette smoke-treated and untreated cells. Altogether 1932 proteins were quantified with at least two unique peptides, with 686 proteins found to be significantly differentially abundant with p > 0.05. Of these proteins the abundance of 64 proteins was at least 2-fold down-regulated after cigarette smoke treatment while 120 proteins were 2-fold up-regulated. The analysis of associated biological processes revealed an alteration of proteins involved in RNA processing and transport as well as extracellular matrix remodelling in response to cigarette smoke treatment.
年龄相关性黄斑变性（AMD）是一种通常影响老年人的病症，会导致视野中心黄斑区的视力丧失。这种疾病的干性形式表现为视网膜色素上皮萎缩，导致视网膜脱离和光感受器丧失。香烟烟雾是干性AMD的一个主要危险因素，会使患该病的风险增加两倍。为了了解香烟烟雾对视网膜色素上皮细胞的影响，将培养的人ARPE - 19细胞用香烟烟雾提取物处理24小时。使用定量质谱法鉴定了3000多种蛋白质，并比较了香烟烟雾处理细胞和未处理细胞中它们各自的丰度。共有1932种蛋白质用至少两种独特肽段进行了定量，发现686种蛋白质的丰度存在显著差异（p＞0.05）。在这些蛋白质中，64种蛋白质的丰度在香烟烟雾处理后至少下调了2倍，而120种蛋白质上调了2倍。对相关生物学过程的分析表明，响应香烟烟雾处理，参与RNA加工和转运以及细胞外基质重塑的蛋白质发生了改变。
