Negrini R, Lisato L, Cavazzini L, Maini P, Gullini S, Basso O, Lanza G, Garofalo M, Nenci I
Istituto di Anatomia e Istologia Patologica, Universitá di Ferrara, Italy.
Gastroenterology. 1989 Feb;96(2 Pt 1):414-20. doi: 10.1016/0016-5085(89)91565-5.
Monoclonal antibodies were raised against Campylobacter pylori to detect the organism in clinical specimens by the immunoperoxidase technique. Twenty-one hybridoma cultures obtained were screened on tissue sections of gastric mucosa to identify antibodies that did not cross-react with host cells. To select antibodies with high grade of specificity to C. pylori, hybridomas were also screened by an enzyme-linked immunosorbent assay against a wide panel of gram-negative bacteria. Fifteen antibodies showed a variable grade of cross-reactivity with gastric mucosa, indicating the presence of at least three epitopes shared by C. pylori and epithelial cells. Three of the six antibodies non-reacting with gastric mucosa (CB-1, CB-26, and CB-31) also were found to be specific for C. pylori. Using CB-26 in an indirect immunoperoxidase test, gastric brushings from 60 patients consecutively undergoing endoscopic examination were examined. By light microscopy, we observed the presence of large amounts of C. pylori in smears from all the 32 patients with positive culture. A small number of bacterial cells were also seen in 8 of 28 patients whose cultures were negative. These findings suggest that the monoclonal antibody CB-26 might be of value for rapid and specific detection of C. pylori.
制备了抗幽门螺杆菌的单克隆抗体，以通过免疫过氧化物酶技术检测临床标本中的该菌。对获得的21种杂交瘤培养物在胃粘膜组织切片上进行筛选，以鉴定不与宿主细胞发生交叉反应的抗体。为了选择对幽门螺杆菌具有高度特异性的抗体，还通过酶联免疫吸附测定针对多种革兰氏阴性菌对杂交瘤进行筛选。15种抗体与胃粘膜表现出不同程度的交叉反应，表明幽门螺杆菌和上皮细胞至少共有三个表位。在六种不与胃粘膜反应的抗体（CB-1、CB-26和CB-31）中，有三种也被发现对幽门螺杆菌具有特异性。使用CB-26进行间接免疫过氧化物酶试验，对60例连续接受内镜检查的患者的胃刷检标本进行检测。通过光学显微镜观察，在所有32例培养阳性的患者涂片标本中均观察到大量幽门螺杆菌。在28例培养阴性的患者中，有8例也发现了少量细菌细胞。这些结果表明，单克隆抗体CB-26可能对快速、特异性检测幽门螺杆菌具有价值。
