Ghassab Sasan, Dulin Jennifer, Bertone Alicia L
Department of Mechanical and Aerospace Engineering.
Department of Veterinary Medicine, The Ohio State University, Columbus, Ohio.
Vet Surg. 2015 Nov;44(8):970-5. doi: 10.1111/vsu.12404. Epub 2015 Oct 2.
To compare clotting efficiency of platelet-rich plasma (PRP) and concentrated platelet-poor plasma (cPPP) to citrated whole blood after activation by autologous thrombin, bovine thrombin, or calcium chloride (CaCl2 ).
Experimental study.
Healthy adult horses (n = 6).
PRP and cPPP were prepared by commercial devices. Using thromboelastography, clotting variables were compared after activation of citrated autologous blood, PRP, and cPPP by autologous thrombin, bovine thrombin, or CaCl2 , respectively.
PRP had the greatest clot strength and quickest clot rate, whereas cPPP had the weakest clot strength, slowest clot rate, and longest clot initiation time. Bovine thrombin resulted in the shortest clot initiation time, quickest clot rate, and was similar to CaCl2 for greatest clot strength. CaCl2 also resulted in the longest clot initiation time and time to reach maximum clot strength. Autologous thrombin resulted in the lowest clot strength.
When combined with either bovine thrombin or CaCl2 , PRP provided the best combinations for clinical use. Autologous thrombin was suboptimal, but could be an autologous alternative for clinical application. As prepared here, cPPP had inefficient clotting, but may be sufficient for plasma spray indications.
比较富血小板血浆（PRP）和贫血小板浓缩血浆（cPPP）在经自体凝血酶、牛凝血酶或氯化钙（CaCl2）激活后与枸橼酸化全血的凝血效率。
实验研究。
健康成年马（n = 6）。
使用商业设备制备PRP和cPPP。采用血栓弹力图，分别比较经自体凝血酶、牛凝血酶或CaCl2激活的枸橼酸化自体血、PRP和cPPP后的凝血变量。
PRP具有最大的血凝强度和最快的血凝速率，而cPPP的血凝强度最弱、血凝速率最慢且凝血起始时间最长。牛凝血酶导致最短的凝血起始时间和最快的血凝速率，并且在最大血凝强度方面与CaCl2相似。CaCl2还导致最长的凝血起始时间和达到最大血凝强度的时间。自体凝血酶导致最低的血凝强度。
当与牛凝血酶或CaCl2联合使用时，PRP为临床应用提供了最佳组合。自体凝血酶并非最佳选择，但可为临床应用提供一种自体替代方案。如此处制备的cPPP凝血效率低下，但可能足以用于血浆喷雾适应证。
