Jahanian-Najafabadi A, Soleimani M, Azadmanesh K, Mostafavi E, Majidzadeh-A K
Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Tasinim Biotechnology Research Center, AJA University of Medical Sciences, Tehran, I.R. Iran.
Res Pharm Sci. 2015 Jan-Feb;10(1):84-9.
Yersinia pestis which is the causative agent of pneumonic plague and distributed in all continents has led to many deaths during the history. Because of its high mortality rate, it must be diagnosed and treated at the earliest time post infection and therefore, rapid diagnostic tests are required. In the present study, we cloned the coding sequence of F1 capsular antigen of the bacteria in the pBAD/gIII plasmid for later expression and purification of the protein to produce poly and monoclonal antibodies against this antigen, and subsequently to develop rapid and efficient diagnostics tools for Y. pestis infections.
鼠疫耶尔森菌是肺鼠疫的病原体，分布于各大洲，在历史上已导致许多人死亡。由于其高死亡率，必须在感染后尽早进行诊断和治疗，因此需要快速诊断测试。在本研究中，我们将该细菌F1荚膜抗原的编码序列克隆到pBAD/gIII质粒中，以便随后表达和纯化该蛋白，以产生针对该抗原的多克隆和单克隆抗体，并随后开发用于鼠疫耶尔森菌感染的快速高效诊断工具。
