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用于在选择性琼脂平板上直接鉴定产耐热直接溶血素副溶血性弧菌菌落的免疫层析检测方法的评估

Evaluation of an immunochromatographic assay for direct identification of thermostable direct hemolysin-producing Vibrio parahaemolyticus colonies on selective agar plates.

作者信息

Kawatsu Kentaro, Sakata Junko, Yonekita Taro, Kumeda Yuko

机构信息

Division of Bacteriology, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan.

Division of Bacteriology, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan.

出版信息

J Microbiol Methods. 2015 Dec;119:4-6. doi: 10.1016/j.mimet.2015.09.020. Epub 2015 Oct 1.

Abstract

We evaluated the utility of an immunochromatographic assay (NH IC TDH) in identifying thermostable direct hemolysin (TDH)-producing Vibrio parahaemolyticus colonies on selective agar plates. The sensitivity of the NH IC TDH assay was 100% (189 samples) and its specificity was 100% (41 samples) compared with the presence of tdh.

摘要

我们评估了免疫层析法(NH ICTDH)在选择性琼脂平板上鉴定产耐热直接溶血素(TDH)的副溶血性弧菌菌落的效用。与tdh的存在相比,NH ICTDH检测的灵敏度为100%(189个样本),特异性为100%(41个样本)。

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