Palumbo A, Jones C, Lightman A, Carcangiu M L, DeCherney A H, Naftolin F
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06510.
Am J Obstet Gynecol. 1989 Jan;160(1):8-14. doi: 10.1016/0002-9378(89)90077-x.
Ovaries from six women with normal menstrual cycles, a follicle wall biopsy specimen from a gonadotropin-stimulated preovulatory ovary, and a corpus luteum of pregnancy were examined by immunohistochemistry for the presence of immunoreactive renin and angiotensin II. Both antisera densely stained thecal and stromal cells (interstitial complex) and luteal cells. Whereas granulosa cells in developing follicles were either unstained or lightly stained, the heavily luteinized granulosa cells of the preovulatory stimulated follicle were strongly positive for immunoreactive renin and angiotensin II. These anatomic findings are consistent with gonadotropin-stimulated local production of both renin and angiotensin II in the human ovary and support the functional roles proposed for the ovarian renin-angiotensin system in follicle development, ovulation, and luteal function and during pregnancy.
对六名月经周期正常的女性的卵巢、促性腺激素刺激的排卵前卵巢的卵泡壁活检标本以及妊娠黄体进行免疫组织化学检查，以检测免疫反应性肾素和血管紧张素II的存在。两种抗血清均使卵泡膜细胞和基质细胞（间质复合体）以及黄体细胞密集染色。发育中的卵泡中的颗粒细胞要么未染色，要么轻度染色，而排卵前受刺激卵泡中高度黄体化的颗粒细胞对免疫反应性肾素和血管紧张素II呈强阳性。这些解剖学发现与促性腺激素刺激下人类卵巢中肾素和血管紧张素II的局部产生一致，并支持所提出的卵巢肾素-血管紧张素系统在卵泡发育、排卵、黄体功能以及妊娠期间的功能作用。
