Kumari Amit, Satyanarayana T, Singh Bijender
Laboratory of Bioprocess Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, 124001, India.
Department of Microbiology, University of Delhi, South Campus, Benito Juarez Road, New Delhi, 110021, India.
Appl Biochem Biotechnol. 2016 Jan;178(1):197-210. doi: 10.1007/s12010-015-1868-8. Epub 2015 Oct 3.
The optimum values of the critical variables determined by the central composite design of response surface methodology (RSM) for maximum phytase production (1881.26 U g(-1) dry mouldy residue (DMR)) by Sporotrichum thermophile are 2.5 % Tween 80, 1.0 % yeast extract and 48 h of incubation period. Phytase production in the mixed substrate (sugarcane bagasse and wheat bran) fermentation enhanced 11.6-fold over the initial production as a consequence of optimization. Phytase titres are sustainable in flasks, trays and column bioreactor (1796 to 2095 U g(-1) DMR), thus validating the model and the process for large-scale phytase production. When the yeast extract was replaced with corn steep liquor (2 % w/v), a sustained enzyme titre (1890 U g(-1) DMR) was attained, making the process cost-effective. Among all the detergents, Tween 80 supported a higher phytase production than others. The enzyme efficiently liberated nutritional components from poultry feed (inorganic phosphate, soluble protein and reducing sugars) in a time-dependent manner.
通过响应面法（RSM）的中心复合设计确定的嗜热枝孢霉产生最大植酸酶产量（1881.26 U g(-1)干发霉残渣（DMR））的关键变量的最佳值为2.5%吐温80、1.0%酵母提取物和48小时的培养时间。由于优化，混合底物（甘蔗渣和麦麸）发酵中的植酸酶产量比初始产量提高了11.6倍。植酸酶滴度在烧瓶、托盘和柱式生物反应器中是可持续的（1796至2095 U g(-1) DMR），从而验证了该模型和大规模生产植酸酶的工艺。当用玉米浆（2% w/v）替代酵母提取物时，获得了持续的酶滴度（1890 U g(-1) DMR），使该工艺具有成本效益。在所有洗涤剂中，吐温80比其他洗涤剂更能支持较高的植酸酶产量。该酶能以时间依赖性方式有效地从家禽饲料中释放营养成分（无机磷酸盐、可溶性蛋白质和还原糖）。
