Ebani Valentina Virginia, Bertelloni Fabrizio, Turchi Barbara, Filogari Dario, Cerri Domenico
Department of Veterinary Science, University of Pisa, Pisa, Italy.
Department of Veterinary Science, University of Pisa, Pisa, Italy.
Asian Pac J Trop Med. 2015 Sep;8(9):714-7. doi: 10.1016/j.apjtm.2015.07.033. Epub 2015 Jul 29.
To determine the prevalence of zoonotic tick-borne bacteria in feeding ticks removed from hunted wild animals.
PCR was executed on DNA extracted from 77 tick pools to detect Anaplasma phagocytophilum, Bartonella spp., Borrelia burgdorferi sensu lato, Coxiella burnetii and Rickettsia spp.
A total of 432 ticks were collected: 30 (6.94%) Haemaphysalis punctata, 72 (16.7%) Dermacentor marginatus and 330 (76.38%) Ixodes ricinus. For each animal one or two pools of 3 ticks of the same species was constituted. Seventy-seven tick pools were examined by PCR: 58 (75.32%) resulted infected and among them 14 (18.18%) showed co-infections. In particular, 29 (37.66%) pools were positive for Bartonella spp., 23 (29.87%) for Anaplasma phagocytophilum, 16 (20.78%) for Rickettsia spp., and 5 (6.49%) for Borrelia burgdorferi s.l. All samples were negative for Coxiella burnetii.
The results demonstrate the presence of several zoonotic tick-borne pathogens in the studied area, and underline the risk of exposure to infections for hunters not only during the outdoor activity, but also when they manipulate hunted animals infested by infected ticks.
确定从被捕猎的野生动物身上采集的正在吸血的蜱中，人畜共患蜱传细菌的流行情况。
对从77组蜱中提取的DNA进行聚合酶链反应（PCR），以检测嗜吞噬细胞无形体、巴尔通体属、伯氏疏螺旋体狭义亚种、贝氏柯克斯体和立克次体属。
共采集到432只蜱：30只（6.94%）微小牛蜱、72只（16.7%）边缘革蜱和330只（76.38%）蓖麻硬蜱。为每只动物构建一到两组，每组由3只同种蜱组成。通过PCR检查了77组蜱：58组（75.32%）被感染，其中14组（18.18%）显示混合感染。具体而言，29组（37.66%）巴尔通体属呈阳性，23组（29.87%）嗜吞噬细胞无形体呈阳性，16组（20.78%）立克次体属呈阳性，5组（6.49%）伯氏疏螺旋体狭义亚种呈阳性。所有样本贝氏柯克斯体均为阴性。
结果表明，在研究区域存在多种人畜共患蜱传病原体，并强调了猎人不仅在户外活动期间，而且在处理被感染蜱虫寄生的被捕猎动物时，都有接触感染的风险。
