Antony V B, Owen C L, Hadley K J
Department of Medicine, Veterans Administration Medical Center, Indianapolis, IN 46202.
Am Rev Respir Dis. 1989 Jan;139(1):199-206. doi: 10.1164/ajrccm/139.1.199.
The development of the pleural inflammatory response to asbestos remains poorly defined. Importantly, the role of the pleural mesothelial cell in recruitment of neutrophils to the pleural space is not known. We hypothesized that rabbit pleural mesothelial cells stimulated by asbestos fibers release chemotactic factor(s) for neutrophils. Primary cultures of rabbit pleural mesothelial cells were established, and their purity verified by the presence of keratin and hyaluronic acid mucin. Mesothelial cells in serum-free media, in the presence of 30 micrograms/ml of crocidolite asbestos, released chemotaxins for neutrophils. This activity was not dependent on the type of asbestos fiber or fiber length. It was dose-dependent until 30 micrograms/ml of asbestos. The chemotactic fractions had the ability to increase both directed and random migration of neutrophils. The chemotactic activity was not present in sonicated fractions of unstimulated mesothelial cells, nor in supernates of asbestos fibers alone. Characterization of the chemotactic activity showed that it was heat stable (56 degrees C per 30 min) and sensitive to digestion with trypsin and papain. On Sephadex G-50 chromatography, it had a molecular weight between 6,000 and 9,000. Production of the chemotactic activity was inhibitable by cycloheximide. These results demonstrate that pleural mesothelial cells can actively synthesize a protein fraction with chemotactic activity for neutrophils. Production of this mesothelial cell-derived chemotactic activity for neutrophils may play an important role in the initiation of the inflammatory response of the pleura to asbestos.
对石棉的胸膜炎症反应的发展仍不清楚。重要的是，胸膜间皮细胞在将中性粒细胞募集到胸膜腔中的作用尚不清楚。我们假设石棉纤维刺激的兔胸膜间皮细胞会释放中性粒细胞趋化因子。建立了兔胸膜间皮细胞的原代培养物，并通过角蛋白和透明质酸粘蛋白的存在来验证其纯度。在无血清培养基中，在存在30微克/毫升青石棉的情况下，间皮细胞释放出中性粒细胞趋化因子。这种活性不依赖于石棉纤维的类型或纤维长度。直到30微克/毫升的石棉，它都是剂量依赖性的。趋化组分有能力增加中性粒细胞的定向和随机迁移。未刺激的间皮细胞的超声破碎组分以及单独的石棉纤维上清液中均不存在趋化活性。趋化活性的表征表明它对热稳定（56℃，30分钟），对胰蛋白酶和木瓜蛋白酶消化敏感。在Sephadex G-50柱层析上，其分子量在6000至9000之间。趋化活性的产生可被环己酰亚胺抑制。这些结果表明胸膜间皮细胞可以主动合成一种对中性粒细胞具有趋化活性的蛋白质组分。这种间皮细胞衍生的中性粒细胞趋化活性的产生可能在胸膜对石棉的炎症反应的起始中起重要作用。
