School of Science, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand.
School of Science, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand.
Food Chem. 2012 May 1;132(1):544-8. doi: 10.1016/j.foodchem.2011.10.066. Epub 2011 Nov 4.
A simple flow injection (FI)-spectrophotometric system for the screening of antioxidant capacity in herbal extracts was developed. The analysis was based on the color disappearance due to the scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical by antioxidant compounds. DPPH and ascorbic acid were used as reagent and antioxidant standard, respectively. Effects of the DPPH concentration, DPPH flow rate, and reaction coil length on sensitivity were studied. The optimized condition provided the linear range of 0.010-0.300mM ascorbic acid with less than 5%RSD(n=10). Detection limit and quantitation limit were 0.004 and 0.013mM, respectively. Comparison of antioxidant capacity in some herbal extracts determined by the FI system and a standard method was carried out and no significant difference was obtained.
建立了一种简单的流动注射（FI）-分光光度法体系，用于筛选草药提取物中的抗氧化能力。该分析基于抗氧化化合物清除 2,2-二苯基-1-苦基肼（DPPH）自由基引起的颜色消失。DPPH 和抗坏血酸分别用作试剂和抗氧化标准。研究了 DPPH 浓度、DPPH 流速和反应盘管长度对灵敏度的影响。优化条件提供了 0.010-0.300mM 抗坏血酸的线性范围，RSD（n=10）小于 5%。检测限和定量限分别为 0.004 和 0.013mM。用 FI 系统和标准方法测定一些草药提取物的抗氧化能力进行了比较，没有得到显著差异。
